利用高速逆流色谱对一株内生真菌代谢物中抗肿瘤活性物质的分离  

Application of high speed counter-current chromatography in isolation of an anti-tumor component originated from an unidentified endophytic fungi

作　　者：胡德军[1] 夏兴[2] 赵凤生[1] 陈代杰[3] 戈梅[2] 

机构地区：[1]上海交通大学药学院,上海200240 [2]上海来益生物药物研究开发中心,上海201203 [3]上海医药工业研究院,上海200040

出　　处：《化工进展》2008年第4期553-557,共5页Chemical Industry and Engineering Progress

基　　金：国家科技部“创新药物与重要现代化”重大专项(2004AA2Z3784)

摘　　要：利用高速逆流色谱法对一株内生真菌活性代谢产物进行了分离。两相溶剂系统为正己烷-乙酸乙酯-甲醇-水(体积比为1∶3∶3∶3),固定相为系统上相,下相为流动相。从内生真菌的代谢物中分离得到一抗肿瘤细胞活性组分。经高效液相色谱分析,其纯度大于99%以上。其化学结构由ESI-MS、1H NMR和13C NMR确认为Cytochalasin N。High speed counter-current chromatography （HSCC） was applied to the separation of anti-tumor components from an unidentified endophytic fungi. A biphasic solvent system composed of n-hexane-ethyl acetate-methanol-water （1 ： 3 ： 3： 3, volume ratio）was used. The bioactive compound purified and collected was analyzed by high-performance liquid chromatography. A white powder with purity above 99% was obtained. The chemical structure of the bioactive compound was identified with ESI-MS, ^1H NMR and ^13C NMR as cytochalasin N.

关 键 词：高速逆流色谱 内生真菌 抗肿瘤 CYTOCHALASIN N 

分 类 号：O657.72[理学—分析化学]