水稻恶苗病菌对苯并咪唑类杀菌剂抗药性分子机理研究初探  

PRELIMINARY RESEARCH ON MOLECULAR MECHANISM OF BENZIMIDAZOL FUNGICIDE RESISTANCE IN FUSARIUM MONILIFORME

作　　者：陆悦健[1] 周明国[1] 叶钟音[1] D.W.Hollomon J.A.Butters 

机构地区：[1]南京农业大学植保系,南京210095 [2]英国布里斯托尔大学LongAshton研究站

出　　处：《菌物系统》1997年第3期235-240,共6页Mycosystema

基　　金：欧洲共同体生物技术中心中英合作课题!CⅡ#-CT93-0013

摘　　要：用真菌β-微管蛋白基因的丰余寡聚核着酸引物B1和B3,扩增了一段871bp的水稻恶苗病菌Fusariummoniliforme的β微管蛋白基因片段,进行了克隆和DNA序列测定,并根据该序列设计了Fmoniliformeβ-微管蛋白基因的特异性测序引物。经过对恶苗病菌对多菌灵具有不同抗性水平菌株的β-微管蛋白基因核着酸序的比较研究,表明Fmoniliforme的β微管蛋白的165,198,200和257位置氨基酸末发生突变,在克隆的片段内也未发现能引起氨基酸改变的核着酸突变。说明该菌对多菌灵产生抗性的分子机理与目前已知的其他真菌有所不同,有待进～步研究。A 871bp fragment of β-tubulin gene from Fusarium moniliforme was amplified by polymerase chain reaction(PCR) using consensus oligo nucleotide pnmers B1 and B3, cloned and sequenced. The specific sequencing primers for F.moniliforme were designed according to the sequence data . The comparison of the the DNA sequences codon 165, 198, 200 and 217 position and no single base-pair point mutations leading to the substitution of one amino acid were found in the cloned fragment of β-tubulin gene.All this showed F . moniliforme has different molecular mechanism of resistance to MBC from other known fungi .

关 键 词：水稻 恶苗病 恶苗病菌 苯并咪唑 抗药性机理 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] S482.2[农业科学—植物保护]