王鸽脑神经细胞原代分离培养与纯化的研究  

The primary cell culture and purification for neuron of King Pigeon

作　　者：王敏[1] 崔雅莉[1] 李文军[1] 徐世文[1] 李术[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《东北农业大学学报》2008年第3期84-87,共4页Journal of Northeast Agricultural University

基　　金：国家自然科学基金面上资助项目(30471278);黑龙江省教育厅科学基金项目(10541029);黑龙江省研究生创新基金(YJSCX2007-0064HLJ)

摘　　要：研究采用7～8日胚龄的王鸽脑组织,采用胰蛋白酶消化法制备神经细胞悬液,接种于包被多聚赖氨酸的6孔培养板中培养,用阿糖胞苷抑制非神经细胞的生长,以寻求一种简单、可行的原代王鸽脑神经细胞培养方法。结果表明,试验成功地进行了原代王鸽脑神经细胞培养、纯化,并保证有足够数量的神经元存活,证明酶消化法原代培养王鸽脑神经细胞是一种可靠、稳定的获得神经元的方法。The 7-8 days King Pigeons embryo was taken, monolayer neurons were digested by trypsin and seed in culture plate coated by polylysine and the growth of other cells except neurons was inhibited by arabinosylcytosin. The result showed that the primary culture of neurons in this way was performed successfully, and the primary cell culture of King Pigeon neurons by the method of enzyme digestion is a reliable way to obstain neurons and with high stability.

关 键 词：王鸽 神经细胞 原代培养 

分 类 号：S836[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]