鹅副粘病毒HN基因在昆虫杆状系统表达及其抗原性鉴定  

Recombinant baculovirus expression of HN gene of GPMV and the antigenicity of the expressed protein

作　　者：藏玉婷[1] 周丽[1] 姜亦飞[1] 王君伟[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《东北农业大学学报》2008年第3期88-91,共4页Journal of Northeast Agricultural University

基　　金：黑龙江省"十五"攻关课题(GB01B503-02)

摘　　要：血凝素-神经氨酸酶蛋白(HN)是鹅副粘病毒诱导体液免疫的重要靶抗原。经pMD18-T-HN阳性质粒扩增了HN基因,亚克隆至昆虫杆状病毒转移载体pBlueBacHis2A,并将其与线性化的杆状病毒共转染对数生长期的sf 9细胞,得到重组杆状病毒rBv-HN,试验表明,HN在昆虫细胞中得到表达,且产物具有抗原性。Haemagglutinin Neuramindase （HN） protein is the important antigen of goose paramyxovirus which can induce humoral immunity. Using a pair of specific primers designed according to the pMD18-T-HN, the HN gene of goose paramyxovirus was amplified by PCR. The PCR product was cloned into pBlueBacHis2A, and then sf9 cells were transfected with the recombinant plasmid. Recombinant baculovirus rBv-HN was obtained. WesternBlotting showed HN is well expressed, and its product have antigenicity.

关 键 词：鹅副粘病毒 HN基因 昆虫杆状系统表达 抗原性 

分 类 号：S835[农业科学—畜牧学] S813[农业科学—畜牧兽医]