腹侧被盖区DA神经元调节睡眠-觉醒机制的探讨  

REGULATION OF DOPAMINE NEURONS IN VENTRAL TEGMENTAL AREA ON SLEEP-WAKEFULNESS

作　　者：王志[1] 林殷利[1] 

机构地区：[1]上海第二医科大学生理教研室,上海200025

出　　处：《生理学报》1997年第2期135-140,共6页Acta Physiologica Sinica

摘　　要：本实验观察了微量注射γ-氨基丁酸（GABA）和5-羟色胺（5-HT）于大鼠中脑腹侧被盖区（VTA）对该部位多巴胺神经元活动的调节及其对睡眠觉醒的影响。实验观察到：VTA注射GABA（25μg）和5－HT（2μg），伏隔核（Acb）内多巴胺（DA）代谢产物——双羟苯乙酸（DOPAC）分别降低到注射前的68．2％（P<0.01）和升高到136．1％（P＜0．01），并相应减少和增加觉醒。双侧Acb注射DA（10μg），也可明显增加觉醒，此作用可被氟哌啶醇（16pmol）阻断，SCH23390不起作用。上述结果揭示：VTA微量注射GABA和5－HT可分别抑制和兴奋该部位的DA神经元，从而可能通过中脑边缘系统引起睡眠和觉醒。The effects of microinjection of γ-aminobutyric acid (GABA) and serotonin (5-HT)into the ventral tegmental area(VTA) on the activity of dopamine neurons in VTA and sleep-wakefulness were studied in rats. It was observed that in aneasthetized rats microinjection of GABA(25μg) and 5-HT(2μg) respectively decreased or increased the level of 3, 4-dihydroxyphenylacetic acid (DOPAC ) to 68. 2% (P<0.01) and to136. 1 % (P<0. 01) of control value in the nucleus accumbens (Acb) . It was also observed that in free moving rats the time of wakefulness was respectively decreased and increased by the two drugs. Furthermore, the time of wakefulness was enhanced following microinjection of dopamine (10 μg) into Acb, an effect that could be blocked by haloperidal (16 pmol), but not by SCH23390 (2 nmol). The above results suggest that bilateral microinjection of GABA and 5-HT into VTA could exert respectively an inhibitory or an excitutory action on the dopamine neurons in VTA, mediating the sleepwakefulness effect probably through the mesolimbic system.

关 键 词：中脑 腹侧被盖区 多巴胺神经元 睡眠-觉醒 

分 类 号：R338.22[医药卫生—人体生理学] Q426[医药卫生—基础医学]