调控桃树抗旱反应的转录因子PpDREB1的克隆及功能鉴定  

Cloning and Functional Identification of Transcription Factor PpDREB1 Regulating Response to Drought Resistance of Prunus persica

作　　者：龚玉梅[1] 王青华[2] 侯玉霞[2] 王豁然[3] 

机构地区：[1]国家林业局科技发展中心,北京100714 [2]中国农业大学理学院,北京100094 [3]中国林业科学院林业研究所,北京100091

出　　处：《宁夏大学学报（自然科学版）》2008年第1期66-70,共5页Journal of Ningxia University(Natural Science Edition)

基　　金：人事部留学回国人员科技活动择优基金资助项目(2005129);引进国际先进农业科学技术"948"基金资助项目(2005-4-40)

摘　　要：通过酵母单杂交技术从桃树中分离到转录因子PpDREB1,GenBank注册号为EF635424.PpDREB1编码蛋白含有一个高度保守的AP2/EREBP结构域,一个核定位信号和一个激活域,属于AP2/EREBP类家族转录因子的新成员.Northern杂交分析表明,PpDREB1在桃树根、茎和叶中都表达,同时,PpDREB1表达被ABA和干旱诱导,PpDREB1 mRNA累积速度较快,高盐和低温也能诱导PpDREB1高效表达.PpDREB1编码蛋白能激活报告基因LacZ的表达,具有明显的转录激活能力.这些结果表明,PpDREB1参与了非生物胁迫的信号转导途径.另外,将PpDREB1基因构建到pCAMBIA1304表达载体CaMV35S启动子下游,通过农杆菌介导转化法将其转入野生型拟南芥细胞中,转基因拟南芥抗旱性增强.A cDNA encoding AP2/EREBP protein were cloned from Prunus persica by yeast one-hybrid system, was referred to PpDREB1 and the GenBank accession number is EF635424. The protein encoded by PpDREB1 has one highly conserved AP2/EREBP domain, one nucleus location signal and one transcription activation domain, and the proteins show a higher similarity with some AP2/EREBP transcription factors with major roles in the stresses, so PpDREB1 may be new members of AP2/EREBP transcription factor family. The analyses of Northern blotting suggest that PpDREB1 was expressed in the roots, stems, leaves of peach, and were induced by ABA and drought, but the mRNA of PpDREB1 highly accumulated when it was induced. Furthermore, PpDREB1 was also induced by cold and salt stresses. In addition, PpDREB1 transcription factor could specially recognize, then activate the expression of LacZ report gene, showing obvious activation ability. It maybe act as one transcription factor with activation capability and be involved in abiotic stresses. In addition, PpDREB1 genes were constructed into the backward position of CaMV35S promoter of pCAMBIA1304 expression vector, and transformed into Arabidopsis thaliana by Agrobacterium tumefaciens. PpDREB1 genes overexpression in the transgenic Arabidopsis thaliana enhanced drought resistance.

关 键 词：桃树 转录因子 抗旱 PpDREB1 

分 类 号：Q943.2[生物学—植物学]