NOV在真皮来源大鼠间充质干细胞中的表达及活性检测  

Expression and activity of NOV in dermis multipotent mesenchymal stem cells in rats

作　　者：刘雁平[1] 李露斯[1] 王涛[2] 

机构地区：[1]第三军医大学西南医院神经内科,重庆400038 [2]第三军医大学西南医院急救部,重庆400038

出　　处：《西南国防医药》2008年第2期157-159,F0003,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：国家自然科学基金资助项目(30400546)

摘　　要：目的:检测肾母细胞瘤过度表达(NOV)基因在真皮来源大鼠间充质干细胞(DMSCs)中的表达及活性。方法:NOV基因重组质粒转染DMSCs,在荧光倒置显微镜下观察转染产物,RT-PCR法检测转染细胞中NOV基因表达。收集NOV基因修饰DMSCs的条件培养液(简称NOV-CM),作用于对照组DMSCs及转染了NOV基因的DMSCs(简称NOV-DMSCs),检测NOV基因及其分泌蛋白在DMSCs分化中的活性作用。结果:NOV重组质粒体外转染入大鼠真皮多能干细胞后,转染细胞中检测到NOV基因。免疫荧光检测结果显示,NOV基因修饰及NOV-CM培养DMSCs时,DMSCs向神经元方向分化的比例增高,且细胞突起较长。结论:NOV基因可在DMSCs中稳定表达,可能具有促进DMSCs向神经元分化的作用。Objective：To observe the expression and the effects of nephroblastoma overexpressed gene （NOV） on differentiation of dermis multipotent mesenchymal stem cells （DMSCs） in rats. Methods： The recombinant plasmid of NOV gene was transfected into DMSCs with liposome. The expression of NOV gene was detected by RT - PCR method. Conditioned media of DMSCs modified by NOV gene （NOV- CM） was collected. DMSCs were cultured in NOV - CM for 10 days. Differentiation of DMSCs was detected by immunofluorescent staining. Results： NOV gene could be well expressed when it was transfected into DMSCs. NOV - CM was found to be helpful for the differentiation of DMSCs to neurons. When differentiation of DMSCs was modified by NOV gene, the number of neurons also increased. Conclusion：The recombinant plasmid of NOV gene could be transfected into DMSCs. NOV maybe promote the differentiation of DMSCs.

关 键 词：NOV基因 真皮 间充质干细胞 分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]