过氧化氢对体外关节软骨细胞损伤效应及丹参干预作用实验研究  

Effects of hydrogen peroxide on injury of cultured cartilage cells from joint and protection of salvia miltiorrhiza

作　　者：程义权[1] 郭振河[1] 杨家辉[1] 余伦红[1] 

机构地区：[1]解放军第161中心医院骨科,湖北武汉430010

出　　处：《西南国防医药》2008年第2期186-189,F0004,共5页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的:观察过氧化氢(H2O2)对体外培养关节软骨细胞的损伤效应及丹参(salvia miltiorrhiza,SM)的干预作用。方法:采用兔原代膝关节软骨细胞培养技术,将培养的软骨细胞随机分为正常组、损伤对照组(损伤组)和丹参组。损伤组用0.1mmol/L过氧化氢(终浓度)制造体外软骨细胞损伤模型,丹参组将不同浓度的丹参(终浓度0.01g/L、0.05g/L、0.25g/L)于过氧化氢损伤前30min加入,分别测定各组细胞活力(MTT法)及细胞凋亡的变化,测定培养液上清中乳酸脱氢酶(LDH)活性及丙二醛(MDA)含量(硫代巴比妥酸法)变化。结果:0.1mmo/L过氧化氢对体外关节软骨细胞有损伤作用,诱导细胞凋亡作用明显;丹参组细胞活性较损伤组显著升高,软骨细胞凋亡率明显下降,培养液上清LDH、MDA水平显著降低,丹参的保护作用呈浓度依赖性。结论:过氧化氢能诱导体外关节软骨细胞凋亡,丹参对过氧化氢造成的软骨细胞损伤具保护作用。Objective：To study the effects of hydrogen peroxide on injury of cultrued cartilage mils from joint and protecnon of salvia miltiorrhiza. Methods：Rabbit chondrocytes from joint were cultured and they were divided into three groups,i,e.the normal group,injury control group and experimental group.In the injury control group,the cultured chrondrochytes were exposed to hydrogen peroxide of 0.1mmol/L.In the experimental group,the cultured chrondrochytes were pre-treated with different concentrations of salvia miltiorrhiza（0.1/L,0.5g/L and 0.25g/L）before they were exposed to hydrogen peroxide of 0.1mmol/L.The proliferation of the cartilage cell of three groups was detected by MTT method,apoptosis of cartilage cell was observod and the contents of LDH and MDA in the supermatant were examined.Results： Hydrogent peroxide of 0.1mmol/L could damage the catilage cell cultured and bad a significant apoptosis induction In the experimental group.the proliferation of cartilage cell was higher and apoptosis and the contents of LDH and MDA were lower than those in the injury control.The protective effect of salvia miltiorrhiza was dose-dependent.Conclusion：Hydrogen peroxide can induce apoptosis in cartilage cells in vitro and salvia miltiorrhiza can protoct the cartilage cells from damage induced by hydrogen peroxide.

关 键 词：骨性关节炎 软骨细胞 丹参 凋亡 过氧化氢 

分 类 号：R681[医药卫生—骨科学]