氟比洛芬干混悬剂中氟比洛芬的含量测定  

Content Determination of Flurbiprofen Dry Suspension by HPLC

作　　者：阳明[1] 黄建耿[2] 雷小光[3] 钟小斌[3] 

机构地区：[1]广西壮族自治区钦州市第二人民医院药剂科,广西钦州535000 [2]华中科技大学同济医学院药学院,湖北武汉430030 [3]广西医科大学第一附属医院,广西南宁530027

出　　处：《中国药业》2008年第7期15-16,共2页China Pharmaceuticals

摘　　要：目的建立高效液相色谱(HPLC)法测定氟比洛芬干混悬剂中氟比洛芬的含量。方法色谱柱为HypersilC18柱(250mm×4.6mm,5μm),流动相为乙腈-0.02mol/L磷酸二氢钾缓冲液(54∶46,三乙胺调pH值至6.0),检测波长为254nm。结果氟比洛芬质量浓度线性范围是2.0～64.0μg/mL(r=0.9999),日内精密度RSD≤0.79%,日间精密度RSD≤1.04%,平均回收率分别为99.38%,100.05%,99.78%。结论HPLC法专属性强,结果准确,适用于氟比洛芬干混悬剂的含量测定。Objective To establish a HPLC method for determining the content of flurbiprofen in flurbiprofen dry suspension. Methods The Hypersil C18 column （250 mm×4. 6 mm,5μm） was used with the mobile phase of acetonitrile： 0.02 mol/L KH2PO4 （54： 46,adjusting pH 6.0 with triethylamine） and the detection wavelength was 254 nm. Results The method had a good linear relationship in the rang of 2. 0 - 64. 0μg/mL （ r = 0. 999 9）. The precision of intra - day and inter - day RSD was ≤0. 79% and ≤1.04% , respectively. The average recovery rate of low,medium and high concentration of flurbiprofen was 99.38%, 100. 05% and 99. 78%,respectively. Conclusion The method is selective and accurate for the content determination of flurbiprofen in flurbiprofen dry suspension.

关 键 词：氟比洛芬 干混悬剂 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学] R971.1