反相高效液相色谱法测定玄麦甘桔颗粒中甘草酸含量  

Determination of Glycyrrhizic Acid in Xuanmaiganjie Granules by RP-HPLC

作　　者：胡世国[1] 罗川萍 李达富 

机构地区：[1]重庆医药高等专科学校,重庆400030 [2]重庆科瑞制药有限责任公司,重庆400060

出　　处：《中国药业》2008年第7期16-17,共2页China Pharmaceuticals

摘　　要：目的建立测定玄麦甘桔颗粒中甘草酸含量的反相高效液相色谱(RP-HPLC)法。方法采用InertsilODS-3色谱柱(250mm×4.6mm,5μm),流动相为甲醇-0.5%醋酸铵溶液-冰醋酸(68∶32∶0.5),流速为1.0mL/min,检测波长为250nm,柱温为室温;并以甘草酸单铵盐为对照品,用外标法测定玄麦甘桔颗粒中甘草酸的含量。结果甘草酸单铵盐对照品质量浓度在38.04～190.2μg/mL范围内与峰面积线性关系良好,线性回归方程A=5753.99C-5951.70,r=0.9999(n=5);平均回收率为99.67%,RSD=0.49%(n=9)。结论RP-HPLC法分离度好,快速、简便,专属性强,重现性好,准确度高,可用于测定玄麦甘桔颗粒中的甘草酸含量。Objective To establish a RP- HPLC method for the determination of glycyrrhizic acid in Xuanmaiganjie Granules,and to analyse the content of glycyrrhizic acid in samples. Methods The Inertsil ODS-3 column was used （250 mm ×4.6 mm,5μm） with methanol- 0.5% ammonium acetate solution-acetic acid glacial （68：32：0.5） as the mobile phase at a flow rate of 1.0 mL/min. The column temperature was 25℃ and the UV detection wavelength was 250 nm. Results The calibration curve for glycyrrhizic acid was in good linearity with the range of 38.04- 190.2μg/mL with the regression equation A =5 753.99C-5 951.70, r=0. 999 9（n=5）. The average recovery rate of glycyrrhizic acid was 99.67%, RSD =0.49% （n =9）. Conclusion The method is simple, sensitive and reproducible.

关 键 词：反相高效液相色谱法 玄麦甘桔颗粒 甘草酸 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]