IGF-1对IL-1诱导的兔关节软骨细胞NO和PGE2的影响  

Effect of IGF-1 on NO and PGE_2 in rabbit articular chondrocytes induced by IL-1

作　　者：彭程[1] 肖涛[1] 罗远明[1] 刘夏君[1] 林绵辉[1] 胡金玺[1] 

机构地区：[1]中南大学湘雅二医院创伤骨科研究室

出　　处：《中南大学学报（医学版）》2008年第3期197-203,共7页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(30571883)~~

摘　　要：目的:检测合成性细胞因子胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)对损伤性细胞因子IL-1(interleukin-1)诱导的兔关节软骨细胞产生NO(nitricoxide)和前列腺素(prostaglandin E2,PGE2)的影响,探讨IGF-1在骨关节炎治疗中的作用机制。方法:实验分为IL-1β10μg/L组、IL-1β10μg/L+IGF-11μg/L组、IL-1β10μg/L+IGF-110μg/L组、IL-1β10μg/L+IGF-150μg/L组、IL-1β10μg/L+IGF-1100μg/L组、IGF-150μg/L组和空白对照组。首先进行2月龄兔原代软骨细胞的培养并进行鉴定,然后将IL-1β10μg/L单独或与不同浓度的IGF-1共育于第2代兔关节软骨细胞,硝酸还原酶法测定实验组细胞上清液NO的含量,ELISA酶联免疫竞争法测定细胞上清液中PGE2含量,再对测定的NO和PGE2的浓度与IGF-1和IL-1的浓度进行有关的统计学分析。结果:IL-1β10μg/L组NO浓度为(89.971±10.224)μmol/L,PGE2浓度为(22.028±8.731)ng/L;空白组NO浓度为(12.404±8.809)μmol/L,PGE2浓度为(1.900±0.227)ng/L。IL-1β10μg/L组与空白组比较,NO和PGE2明显增加,差异有统计学意义(P<0.05)。在IL-1β均为10μg/L时,IGF-1可以呈剂量依赖地降低IL-1诱导的兔关节软骨细胞NO和PGE2的升高,并且在50μg/L时即可达到最佳浓度。结论:IL-1能增加软骨细胞培养中的NO和PGE2的产生。IGF-1在体外可以呈剂量依赖地降低IL-1诱导的兔关节软骨细胞NO和PGE2的升高,其最佳浓度为50μg/L。Objective To explore the effect of insulin-like growth factor （ IGF-1 ） on the concentration of NO and PGE2 in the supernatant of rabbit articular chondrocytes induced by IL-1, and to explore the mechanism of IGF-1 in the development of osteoarthritis （ OA ）. Methods The sam- ples were divided into 7 groups ： IL-1β 10μg/L group, IL- 1β 10μg/L ＋ IGF-1 1 μg/L group, IL-1β 10μg/L ＋ IGF-1 10 μg/L group, IL-1β 10 μg/L ＋ IGF-1 50 μg/L group, IL-113 10 μg/L ＋ IGF-1 100 μg/L group, IGF-1 50 μg/L group, and a blank control group. The chondrocytes from the articular cartilage of 2 month old rabbits were cultivated and identified,and then cocultured in the second filial generation chondrocytes on plates with or without recombinant human IGF-1 or IL-1.The concentration of NO was detected by nitrate reductase kit,and that of PGE2 by enzyme-linked immunosorbent assay（ELISA）.The results were analyzed by statistical method.Results The average value of NO and PGE2 was （89.971±10.224）μmol/L and （22.028±8.731）μmol/L in the IL-1β 10μg/L group,and （12.404±8.809）μmol/L and （1.900±0.227）ng/L in the blank control group.The concentration of NO and PGE2 in IL-1β 10μg/L group was significantly higher than that in the blank control group （P〈0.05）.At the same concentration of 10μg/L,iGF-1 could dose-dependently decrease the increase of NO and PGE2 concentration induced by IL-1β in the chondrocytes supernatant in vitro,and the optimum concentration of IGF-1 was 50μg/L.Conclusion IL-1 can significantly increase the concentration of NO and PGE2,and IGF-1 an dose-dependently decrease the concentration of NO and PGE2 in the chondrocytes supernatant in vitro.The optimum concentration of IGF-1 was 50μg/L.

关 键 词：白介素-1 胰岛素样生长因子-1 一氧化氮 前列腺素E2 骨关节炎 软骨细胞 

分 类 号：R684.3[医药卫生—骨科学]