软骨细胞在纳米级PHBV支架材料与PHBV/ECOFLEX复合支架材料中的生长状况比较  

Growth of chondrocytes in nano-structured PHBV scaffold and PHBV/ECOFLEX composite scaffold

作　　者：陈敏洁[1] 杨驰[1] 俞昊[2] 蒋兴泉 朱美芳[2] 姜滨[1] 张秀丽 

机构地区：[1]上海交通大学医学院附属第九人民医院.口腔颌面外科,上海200011 [2]东华大学材料科学与工程学院纤维材料改性国家重点实验室,上海200051 [3]上海市口腔医学研究所,上海200011

出　　处：《中国口腔颌面外科杂志》2008年第2期119-123,共5页China Journal of Oral and Maxillofacial Surgery

基　　金：上海市科学技术委员会纳米专项(0452nm006)~~

摘　　要：目的:比较纳米级PHBV与PHBV/ECOFLEX复合支架材料在体外培养实验中对软骨细胞的影响,从而评价其作为软骨组织工程支架材料的可行性。方法:在纳米级PHBV聚集体与PHBV/ECOFLEX复合物上分别接种羊耳郭软骨细胞,并进行体外培养,取4h、24h、2d、4d、7d标本进行扫描电镜和组织学观察,评价软骨细胞的生长状况。结果:电镜及组织学观察均显示,纳米级PHBV支架材料中软骨细胞脱落较少,软骨细胞在支架表面黏附、铺展,并分泌细胞外基质;而PHBV/ECOFLEX复合材料塌陷明显,软骨细胞脱落较多,软骨细胞在支架表面未铺展。但2种支架材料内部的软骨细胞均较少。结论:纳米化处理的PHBV对软骨细胞有明显的吸附、诱导作用,可作为组织工程中的软骨修复支架材料。PURPOSE： To compare the potential use of nano-structured PHBV and PHBV/ECOFLEX composite as tissue engineering scaffold materials for chondrocyte regeneration. METHODS： Chondrocytes isolated from auricular cartilage of goat were seeded onto the surfaces of nano-structured PHBV and PHBV/ECOFLEX composite scaffolds. After culturing in vitro for 4 hours, 24 hours, 2 days, 4 days and 7 days, the chondrocytes-PHBV constructs were respectively examined histologically and under scanning electron microscope. RESULTS： The adhesion, growth and formation of extrocellular matrix on nano-structured PHBV were significantly better than those on PHBV/ECOFLEX composite. Chondrocytes inside the nano-structured PHBV were not found. CONCLUSIONS： Nano-structured PHBV is much better than PHBV/ECOFLEX composite as scaffold in cartilage tissue engineering in vitro system. Supported by Nano-Fund of Science and Technology Committee of Shanghai Municipality（Grand No. 0452nm006）.

关 键 词：纳米级PHBV PHBV/ECOFLEX 支架 软骨细胞 

分 类 号：R318.08[医药卫生—生物医学工程]