中国北方汉族人载脂蛋白E基因多态性与血脂代谢的关系  

Relationship between polymorphisms of apolipoprotein E gene and serum lipids in Northern Chinese Han population

作　　者：容春莉[1] 彭应心[1] 刘美霞[1] 王秀萍[1] 宋津晓[2] 

机构地区：[1]河北省人民医院心脏中心二科,河北石家庄050051 [2]河北省人民医院急救中心,河北石家庄050051

出　　处：《中国现代医学杂志》2008年第5期586-588,591,共4页China Journal of Modern Medicine

摘　　要：目的从基因水平探讨载脂蛋白E(ApoE)基因多态性与中国北方汉族人血脂的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术测定150例心血管病患者(病例组)和130例正常对照者(对照组)的ApoE基因多态性,并测定血脂代谢的指标,研究ApoE基因多态性与血脂水平的关系。结果在3种等位基因间,总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)及载脂蛋白B(ApoB)水平均按ε4基因携带者>ε3基因携带者>ε2基因携带者的顺序递减,差异有显著性(对照组均P<0.05,病例组均P<0.01),而三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、载脂蛋白AI(ApoAI)水平差异无显著性(均P>0.05)。结论ApoE基因多态性影响中国北方汉族人的血脂代谢,ε4等位基因对TC、LDL-C及ApoB有升高作用。[Objective] To study the relationship between polymorphisms of apolipoprotein E（ApoE） gene and serum lipids in Northern Chinese Han population by gene level.[Methods] ApoE gene polymorphism was genotyped in 150 cardiovascular patients （patient group） and 130 healthy controls （control group） by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method. Meantime, the levels of serum lipids were measured. [Results]In comparison of the levels of serum lipids among three kinds of Apo E allele, the levels of TC, LDL-C and ApoB were decreased progressively according to ε4 allele 〉ε3 allele 〉ε2 allele, which had a statistically difference （all P 〈0.05 in control group, all P 〈0.01 in patient group）, while the levels of TG, HDL-C and ApoAI had no significant differentce among the three groups （all P 〉0.05）. [Conclusions] ApoE gene polymorphism affects serum lipid metabolism in Northern Chinese Han population, and ε4 allele has a function of increasing TC,LDL-C and ApoB.

关 键 词：载脂蛋白E 基因多态性 聚合酶链反应-限制性片段长度多态性技术 血脂 

分 类 号：R349.7[医药卫生—基础医学]