细胞内外膜跨膜电位频率响应模型及滤波器特性的研究  

Frequency Response Model of Transmembrane Potential on Cellular Inner and Outer Membrane and its Filter Characteristic Simulation

作　　者：姚陈果[1] 莫登斌[1] 孙才新[1] 米彦[1] 李成祥[1] 廖瑞金[1] 

机构地区：[1]重庆大学电气工程学院输配电装备及系统安全与新技术国家重点实验室,重庆400044

出　　处：《中国生物医学工程学报》2008年第2期270-275,共6页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金项目(50637020;50407007);教育部博士点基金(20050611020);重庆市科技攻关重大专项(2005AA5008-3)

摘　　要：提出球形单细胞内外膜跨膜电位频率响应模型和仿真计算方法。通过对频率响应的仿真分析,发现内外膜跨膜电位分别表现出一阶带通和低通滤波器特性,并在内膜频率响应中心频率到上限截止频率的范围内,内膜跨膜电位大于外膜同时能保持在较高水平,这将有利于诱导细胞内电处理效应。计算结果与动物实验吻合得很好,为纳秒脉冲电场治疗肿瘤的窗口参数合理选择提供了理论依据。The frequency response model and simulation method of transmembrane potential on inner and outer membrane of spherical biological cell was presented in this paper. By simulation and analysis of the frequency response, it was found that transmembrane potential on inner membrane showed first-order band-pass filter characteristic, while that outer membrane represented first-order low-pass filter characteristic. Moreover, transmembrane potential on inner membrane exceeded that on outer membrane and kept a higher value in the range from center frequency to upper cut-off frequency, which was favorable to induce intracellular electromanipulation. The calculation result agrees well with the data from animal experiment, which provided a theoretical guidance to choosing reasonable window parameters for application of nanosecond pulse to tumor treatment.

关 键 词：频率响应 跨膜电位 细胞内外膜 滤波器特性 模型 

分 类 号：Q64[生物学—生物物理学]