东方田鼠肺脏裂解物筛选日本血吸虫成虫噬菌体展示cDNA文库  

SCREENING A PHAGE DISPLAY CDNA LIBRARY OF SCHISTOSOMA JAPONICUM WITH EXTRACT FROM LUNG OF MICROTUS FORTIS

作　　者：孙焕[1] 刘金明[1] 孙帅[1] 宋震宇[1] 石耀军[1] 陆珂[1] 李浩[1] 金亚美[1] 林矫矫[1] 

机构地区：[1]中国农业科学院上海兽医研究所/国家防治动物血吸虫病专业实验室/农业部动物寄生虫学重点开放实验室,上海200232

出　　处：《中国兽医寄生虫病》2008年第2期5-9,共5页Chinese Journal of Veterinary Parasitology

基　　金：上海市科学技术委员会科研计划项目(054909002);科技部自然资源平台项目(2005DKA21104)

摘　　要：目的寻找与东方田鼠抗性相关的血吸虫靶基因。方法以东方田鼠肺脏裂解物为探针,亲和淘洗筛选日本血吸虫成虫(44 d)噬菌体展示cDNA文库。三轮筛选后,随机挑取76个阳性噬菌体克隆进行序列测定及生物信息学分析。结果共获得了13个有效EST序列,其中8条EST序列与已知基因或表达序列标签同源,5个EST序列与已知基因或表达序列标签均无同源性,为新的表达序列标签。功能预测结果显示,这13个有效EST编码蛋白主要与血吸虫基因表达调控及蛋白质加工修饰相关。结论东方田鼠直接或间接地影响血吸虫基因表达,进而影响血吸虫的发育,可能是东方田鼠具有抗血吸虫特性的一个重要分子机理。Objective To find out the genes of Schcistosoma japonicum （ S. japonicum） related to the Schistosoma-resistance of Microtus fortis（M, fortis）. Methods A phage display cDNA library from worms of S. japonicum was screened with lung extraction of M. fortis. After three rounds of biopanning, 76 positve clones picked at random were sequenced and functional analysed through bioinformatics. Results Thirteen ESTs including 5 unreported ESTs were obtained. Function prediction indicated that these ESTs were mainly involved in the regulation of gene expression and protein modification of Schistosoma japonicum. Conclusion M. fortis could influece the gene expression of Sjaponicu and hinder the development of the parasite in its body, which might be an important mechanism of Schistosoma-resistance of M. fortis.

关 键 词：日本血吸虫 东方田鼠 筛选 噬菌体展示cDNA文库 

分 类 号：S858.912.735[农业科学—临床兽医学]