家兔的超数排卵  

STUDIES ON SUPEROVULATION IN RABBITS

作　　者：李子义[1,2] 周琪[1,2] 文兴豪 岳占碰[1,2] 孙兴参[1,2] 刘忠华[1,2] 李武 谭景和[1,2] 

机构地区：[1]东北农业大学生物工程系 [2]解放军农牧大学基础部

出　　处：《东北农业大学学报》1997年第3期275-280,共6页Journal of Northeast Agricultural University

基　　金：黑龙江省科委资助

摘　　要：应用国产激素制剂对大耳白、青紫兰和比利时３种家兔进行了超数排卵处理，系统研究了品种、激素剂量、发情周期、重复超排间隔时间以及体重、季节等因素对超排效果的影响。实验结果表明，１）ＰＭＳＧ和ｈＣＧ对３种家兔超排处理的最适剂量有差异，大耳白兔为１３０ＩＵ剂量组最好（平均获卵２４．３８±５．５７个），青紫兰兔为１１０ＩＵ剂量组最佳（平均获卵２６．００±４．３１个），而比利时兔的剂量效应不明显（１３０ＩＵ剂量组好于其他各组，平均获卵１６．００±２．３５个）；２）同种家兔间隔１～２个情期与间隔３～４个情期重复超排效果差异不显著（Ｐ＞０．０５），且分别与各自初次超排效果相比也相差不显著（Ｐ＞０．０５）；３）发情周期不同阶段开始超排效果不同，大耳白兔和青紫兰兔发情早期至发情晚期的超排效果显著（Ｐ＜０．０５）或极显著（Ｐ＜０．０１）好于间情期；４）以１２０ＩＵ～１３０ＩＵ剂量进行超排处理，体重在２．００～２．９９ｋｇ的青紫兰兔的超排回收卵数为２６．３８±７．４７个，极显著多于体重在４．００～４．９９ｋｇ的青紫兰兔（获卵１３．７５±４．８５个）（Ｐ＜０．０１）；５）大耳白兔在１０～１２月份的超排回收卵数为２５．７３±６．２８个?The present study was carried out to establish an effective method for the superovulatory treatment of rabbits with Chinese-made PMSG and hCG White, Chinchilla and Belgium rabbits were used in this study Several factors affeting superovulation such as breed, hormone dosage, interval of repeated superovulation, weight and season were systematically studied The results obtained were as follows: 1) The best dosages for superovulation were 130 IU in White rabbits (oocytes recovered: 24 38±5 57), 110 IU in Chinchilla rabbits (oocytes recovered: 26 00±4 31) and 130 IU in Belgium rabbits (oocytes recovered: 16 00±2 35) 2) The difference in ovulation rates was not significant ( P >0 05) between superovulation and repeated superovulations at intervals of 1～2 estrous cycles or 3～4 estrous cycles 3) The ovulation rate was significantly ( P <0 05 or 0 01) different when superovulatory treatment was started at proestrus to metestrus from when it was started at diestrus in White rabbits and Chinchilla rabbits 4) Under the dosage of 120 IU～130 IU, the effect of weight on superovulation was highly significant ( P <0 01) in Chinchilla rabbits 5) The effect of seasonal variation on superovulation was significant ( P <0 05) in White rabbits and Chinchilla rabbits

关 键 词：超数排卵 激素剂量 品种 情期 体重 季节 家兔 

分 类 号：S829.1[农业科学—畜牧学] S814.4[农业科学—畜牧兽医]