IMPACTS ON THE EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN ISCHEMIC BRAIN TISSUE TREATED WITH CLUSTER PUNC-TURE ON SCALP ACUPOINTS IN RATS  

头穴丛刺对大鼠缺血脑组织血管内皮生长因子表达的影响(英文)

作　　者：李宝栋[1] 王志勇[1] 白晶[2] 白海娟 公维志[4] 闫瑞萍[1] 刘振江[1] 王利春[1] 

机构地区：[1]河北省沧州中西医结合医院神经内科 [2]黑龙江中医药大学研究生院 [3]内蒙古自治区牙克石市卫生监督所 [4]黑龙江中医药大学附属第二医院针灸推拿科

出　　处：《World Journal of Acupuncture-Moxibustion》2008年第1期38-44,共7页世界针灸杂志（英文版）

基　　金：河北省中医药管理局项目,编号:05088~~

摘　　要：Objective To probe into the impacts on the expression of vascular endothelial growth factor （VEGF） in ischemic brain tissue treated with cluster puncture on scalp acupoints in rats, Methods 128 rats were randomized into pseudo-operation group, model group, scalp-needling group and cluster-needling group, In scalp-needling group, penetration method was used on the focal side from Bǎihuì （百会 GV20） to Qǔbīn （曲鬓 GB7）, and in cluster-needling group, penetration method was used on both sides from GV20 to GB7, and a common needling on GV 20. Needles were punctured 2 mm in depth, constantly rotated for 10min, retained for 2 h. Immunohistrochemical method was applied to determine VEGF expression and microvessel density （MVD）. Results With the intervention of cluster puncture on scalp acupoints, VEGF： expressions on every time-spot after ischemia were enhanced apparently, superior to those in scalp-needling group. On the three time-spots of the 7^th, 14^th and 21^st days, MVD was increased after cluster puncture on scale acupoints, superior to those in scalp-needling group. Conclusion Cluster puncture on scalp acupoints up-regulated VEGF expression and promoted regeneration of microvessel.目的:探讨头穴丛刺法对大鼠缺血脑组织血管内皮生长因子(VEGF)表达的影响。方法:128只大鼠随机分为假手术组、模型组、头针组和头穴丛刺组;头针组大鼠取病灶侧百会透曲鬓,头穴丛刺组大鼠双侧百会透曲鬓及百会穴;均进针2mm,持续捻转10 min,留针2 h。以免疫组化法测定血管内皮生长因子的表达,测定微血管密度。结果:在缺血后各时间点,经头穴丛刺干预后的VEGF表达明显增强,优于头针组;在7d、14d、21d三个时间点,经头穴丛刺干预后微血管密度增多,优于头针组。结论:头穴丛刺法可以通过促使VEGF表达上调,促进微血管新生。

关 键 词：Cluster puncture on scalp acupoints Rat Ischemia Vascular endothelial growth factor （VEGF） 

分 类 号：R245[医药卫生—针灸推拿学]