蓖麻蚕核型多角体病毒解旋酶基因的克隆和序列分析  

Cloning and Sequence Analysis of helicase Gene of Philosamia cynthia ricini nucleopolyhedrovirus

作　　者：陈言[1] 张月华[2] 李兵[3] 朱姗颖[1] 徐安英[2] 王文兵[1] 沈卫德[3] 

机构地区：[1]江苏大学生命科学研究院,江苏镇江212013 [2]中国农业科学院蚕业研究所,江苏镇江212018 [3]苏州大学生命科学学院,江苏苏州215123

出　　处：《蚕业科学》2008年第1期65-71,共7页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划“973”项目(编号2005CB121-005);江苏省高技术研究(农业)项目(编号BG2007322);江苏省高校自然科学指导性计划项目(编号06KJD180043)

摘　　要：为了解柞蚕核型多角体病毒(Antheraea pernyinucleopolyhedrovirus,ApNPV)和蓖麻蚕核型多角体病毒(Philosamia cynthia riciniNPV,PcrNPV)在感染性及基因水平上的差别,克隆了PcrNPV解旋酶基因helicase,对该基因进行了测序及同源性分析。感染性试验表明,ApNPV对柞蚕和蓖麻蚕的感染率分别为78%和57%;而PcrNPV对蓖麻蚕的感染率为79%,但几乎不感染柞蚕。对PcrNPVhelicase分析的结果表明,该基因长度为3 639 bp,编码1 212个氨基酸(登录号:EU143371)。基因的同源性分析表明,PcrNPVhelicase与ApNPVhelicase的同源性最高,达98.6%,与苜蓿银纹夜蛾NPV(Autographa califorlicaNPV,AcNPV)helicase和家蚕NPV(BombyxmoriNPV,BmNPV)helicase的同源性最低,分别为58%和58.8%。PcrNPV和ApNPV的helicase基因7个保守功能域上的氨基酸序列均相同,仅在靠近DNA结合区螺旋-转角-螺旋结构处的第974位上有1个氨基酸不同,推测该位点可能与宿主域范围有关。In order to identify the difference on the infection and genes between Antheraea pernyi nucleopolyhedrovirus （ApNPV） and Philosamia cynthia ricini NPV （ PcrNPV）, we carried out these experiments. The results of infection experiments indicated that ApNPV can infect both Antheraea pernyi and Philosamia cynthia ricini larvae, with the infectivity of 78% and 57%, respectively. However, PcrNPV can infect Philosamia cynthia ricini, with the infectivity of 79% and had almost no infectivity to the larva of Antheraea pernyi. The cloned PcrNPV heficase gene was 3 639 bp long and encoded a protein of 1 212 amino acids （Aceession number. EU143371 ）. The homologous analysis results showed that PcrNPV heficase had the highest identity （98.6%） to that of ApNPV, and had the lowest identity to that of Autographa cafiforlica multiple NPV （AcMNPV） and Bombyx mori NPV （BmNPV）, with the identity of 58% and 58.8%, respectively. The seven conserved motifs of ApNPV and PcrNPV helicase gene were completely ideutical, while only at the amino acid position 974, a different amino acid near the la motif and helix-turn-helix motif was found,suggesting that this position might be related with the host range.

关 键 词：蓖麻蚕核型多角体病毒 柞蚕核型多角体病毒 解旋酶基因 序列分析 

分 类 号：S885.2[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]