花生FAD2-RNAi载体基因AFLP标记的克隆及序列分析  

Molecular Cloning and Sequence Analysis of △^(12)-fatty Acid Desaturase-RNAi Vector Based AFLP in Peanut

作　　者：张宁洁[1] 高国庆[2] 熊发前[2] 

机构地区：[1]广西大学,广西南宁530003 [2]广西农业科学院生物实验室,广西南宁530003

出　　处：《花生学报》2008年第1期7-13,共7页Journal of Peanut Science

基　　金：广西自然科学基金项目(桂科自0342003)

摘　　要：以花生高油酸品种Sunolic95R和低油酸品种汕油162杂交组合的F1为试验材料,采用AFLP分子标记,用100对AFLP引物筛选出20个与△12-脂肪酸脱氢酶-RNAi载体基因遗传连锁距离为3.87~8.91cm的差异片段,并回收纯化、克隆测序及NCBI上进行序列比对,最终获得了5个FAD2-RNAi载体基因的全序列(长度为589~763bp),通过分析得出:所获得的5个FAD2-RNAi载体基因的遗传连锁距离为3.87~6.59cm,序列间同源性达到88%~97%,与GenBank中已知的FAD2-RNAi载体基因序列相似性均达到85%以上,分别包含一个起始密码子与一个终止密码子,结果表明本试验采用AFLP技术获得的5个序列均为△12-脂肪酸脱氢酶-RNAi载体基因。The F1 of high oleic acid Sunolic95R and low oleic acid San162 was used as experimetal material. Nearly one hundred of primers are boltinged with AFLP. We obtained twenty AFLP mark- ers which have labeled between 3.87cm and 8.91cm with A12-fatty acid desaturase-RNAi vector. Five sequences are obtained after recover, isogenic, clone and measure and were compared by blastn in the GenBank. The analytic result showed the five sequence were labeled between 3.87cm and 6.59cm. the identity among them was 88%-97% and above 87% with cited sequences. These analyses revealed the obtained sequence were A12-fatty acid desaturase-RNAi vector.

关 键 词：花生 油酸 FAD2-RNAi载体 序列分析 

分 类 号：S565.202.4[农业科学—作物学]