HPLC法测定复方芦丁片中芦丁的含量  

Determination of Rutin in Compound Rutin Tablets by HPLC

作　　者：林远凤[1] 黄燕萍[2] 

机构地区：[1]广西北海市人民医院,536000 [2]广西北海食品药品检验所

出　　处：《黑龙江医药》2008年第2期8-9,共2页Heilongjiang Medicine journal

摘　　要：目的:建立高效液相色谱法测定复方芦丁片中芦丁的含量。方法:以 Warters Nova-pak(?)C_(18)(3.9×150mm,4um)为色谱柱;以乙腈-四氢呋喃-0.2%枸橼酸溶液(14:1:85)为流动相;流速为1.0ml·min^(-1);检测波长为360nm。结果:芦丁在0.8372～5.0232μg(r=0.9999)范围内与峰面积呈良好的线性关系;平均回收率为99.05%,RSD 为0.71%。结论:本法快速、准确,可用于测定复方芦丁片中芦丁的含量。Objective： To establish a HPLC method for determination of rutin in Compound Rufin Tablets by HPLC. Methods： HPLC was used with Waters Nova - pak C18 （3.9 × 150mm, 4um） colunm; acetonitrile - tetrahydrofuran - 0.2%citric acid（14： 1：85）was used as mobile phase.The flow rate was 1.0 ml · min^-1;The detection wavelength was at 360 nm.Results： The linear response range lay in 0.8372-5. 0232μg of rutin（ r = 0.9999）.The average recoveries of rutin was 99.05 %, RSD = 0.71%. Conclusion：This method is rapid, accurate and suitable for the determination of the compound tablets.

关 键 词：高效液相色谱法 芦丁 复方芦丁片 

分 类 号：R927.2[医药卫生—药学]