HLA-GmRNA差异表达对细胞膜表面HLA-G异构体分子表达的影响  

Effect of different HLA-G isoforms mRNA on its cell membrane expression

作　　者：颜卫华[1] 林爱芬[1] 许惠惠[1] 陶丹丹[1] 

机构地区：[1]温州医学院附属浙江省台州医院,临海317000

出　　处：《中华微生物学和免疫学杂志》2008年第3期250-253,共4页Chinese Journal of Microbiology and Immunology

基　　金：浙江省自然科学基金（Y205531,Y205575）;人事部留学回国人员择优项目（浙人专[2005]188）

摘　　要：目的探讨HLA-G异构体（HLA-G1-6）mRNA差异表达对HLA-G分子在细胞表面表达的影响。方法通过RT．PCR方法分析卵巢癌细胞株HO-8910、HO-8910PM、OVCAR-3，白血病细胞株Jurkat、K562、HL60、MUTZ-1，绒癌细胞株JEG-3、JAR内HLA-G异构体mRNA的表达种类，采用流式细胞术分析上述细胞株细胞表面及细胞内HLA—G分子的分布及表达水平。结果阳性对照JEG-3细胞内表达HLA-G1—6mRNA，阴性对照JAR不表达HLA-G1—6mRNA。HLA—G1mRNA在HO-8910、HO．8910PM、OVCAR-3、MUTZ-1、Jurkat细胞内表达。除阳性对照JEG-3外，其他细胞均不表达HLA-G2mRNA；表达HLA-G3 mRNA的细胞有HO-8910、HO-8910PM、K562、HL60、MUTZ-1、OVCAR-3、Jurkat；表达HLA-G4mRNA的细胞有HO-8910、HO-8910PM、HL60、Jurkat；表达HLA-G5mRNA的细胞为Jurkat。FACS分析显示在JEG-3、HO-8910PM、Jurkat细胞膜表面表达HLA-G分子，而除JAR细胞外，其他细胞内均表达HLA-G分子。结论HLA—G2、-G3、-G4、-G5、-G6均不能在细胞表面表达，而HLA—G1分子则能在特定的细胞表面表达。Objective To investigate the mechanism of different HLA-G isoform mRNA patterns in different cells alters its cell membrane expression. Methods RT-PCR was used to analyze HLA-G isoform mRNA （HLA-G1-6） of ovarian cancer cell lines HO-8910, HO-8910PM and OVCAR-3, leukemia cell lines Jurkat, K562, HL60, MUTZ-1, and the choriocarcinoma cell lines JEG-3, JAR. HLA-G between cellular membrane and intracellular expression were analyzed by flow cytometry. Results All HLA-G mRNA isoforms were observed in the positive control cell line JEG-3, but none in the negative control cell line JAR. HLA-G1 isoform mRNA was expressed in HO-8910, HO-8910PM, OVCAR-3, MUTZ-1 and Jurkat cells. HLA-G2 mRNA was not detected in any cell line but JEG-3. HLA-G3 mRNA was found in HO-8910, HO- 8910PM, K562, HL60, MUTZ-1, OVCAR-3 and Jurkat cells. HO-8910, HO-8910PM, HL60, Jurkat cells expressed HLA-G4 mRNA. Only the Jurkat cells expressed HLA-G5 mRNA. FACS results showed that JEG- 3 and HO-8910PM cells membrane expressed HLA-G, however, the intracellular HLA-G expression was detected in all tested cells except the negative control cell JAR. Conclusion Only the HLA-G1 isoform could be expressed on cell membrane in particular cell lines. Other isoforms including HLA-G2, -G3, -G4, -G5 and HLA-G6 could not reach cell surface.

关 键 词：HLA-G MRNA 异构体 细胞株 

分 类 号：R686[医药卫生—骨科学]