血浆中羟乙膦酸钠含量测定方法研究  

Study on Determination of Disodium Etidronate in Plasma

作　　者：邵青[1] 李士敏[1] 郑琦 曾苏[1] 

机构地区：[1]浙江医科大学药学系分析中心

出　　处：《中国药科大学学报》1997年第4期222-224,共3页Journal of China Pharmaceutical University

摘　　要：利用羟乙膦酸（ＥＨＤＰ）与磷酸钙形成共沉淀收集血浆中羟乙膦酸钠，再使无机磷形成不溶性三乙胺磷钼酸络合物而除去。采用高氯酸解离ＥＨＤＰ的Ｐ－Ｃ－Ｐ键使转化为无机磷，钼蓝－乙酸丁酯萃取比色法测定释放的无机磷。结果表明：方法平均回收率为（１０２．９７±４．９３）％，ｎ＝５，精密度试验ＲＳＤ为３．２１％，ｎ＝７，线性范围为０．５１６～１５．４８μｇ／ｍｌ（ｒ＝０．９９９２）。This paper introduces a method on determination of disodium etidronate(EHDP) in plasma The procedure is based on a coprecipitation of EHDP with calcium phosphate, elimination of inorganic phosphate as an insoluble triethylamine phosphomolybdate complex, decomposition of the P-C-P bond with perchloric acid and molybdate blue butylacetate abstraction colorimetry determination of the inorganic phosphate released The results showed that the average recovery was 102 97%±4 93%, and n=5 , precision RSD=3 21%, and n =7 The linear range of the method was 0 516￣15 48 μg/ml

关 键 词：羟乙膦酸钠 血药浓度 

分 类 号：R977.7[医药卫生—药品] R927.2[医药卫生—药学]