去乙酰基壳聚糖在亲和层析纯化胰蛋白酶中的应用  

Application of Chitosan in Purifying Trypsin with Affinity Chromatography

作　　者：孙福璋[1] 金海洙[1] 

机构地区：[1]烟台大学生物化学系

出　　处：《中国药科大学学报》1997年第4期241-243,共3页Journal of China Pharmaceutical University

摘　　要：首次用环氧氯丙烷活化的去乙酰基壳聚糖（Ｃｈｉｔｏｓａｎ）为载体，鸡卵粘蛋白（Ｏｖｏｍｕ－ｃｏｉｄ，简称ＣＨＯＭ）为配基，制备了胰蛋白酶（Ｔｒｙｐｓｉｎ）的亲和吸附剂，纯化效率与琼脂糖（Ｓｅｐｈａｒｏｓｅ－４Ｂ）相近，为４７倍，纯化的Ｔｒｙｐｓｉｎ经ＳＤＳ－ＰＡＧＥ电泳为单一区带。表明Ｃｈｉｔｏｓａｎ可以代替Ｓｅｐｈａｒｏｓｅ－４Ｂ作为亲和层析的载体。With Chitosan activated by epichlorohydrin as carrier and with ovomucoid as ligand the affinity adsorbent for purification of trypsin was prepared for the first time The efficiency of this adsorbent in purifying Trypsin was close to Sepharose 4B affinity adsorbent, and the purification ratio is 47 times Trypsin purified in this way gave only one peak in SDS PAGE analysis All of the experimental results mentioned above indicated that Chitosan could replace Sepharose 4B in affinity chromatography

关 键 词：去乙酰基壳聚糖 胰蛋白酶 亲和层析 

分 类 号：Q503[生物学—生物化学] Q556.3