创伤性深静脉血栓形成中补体相关基因表达变化的实验研究  

Study the genes expressions related to complement of traumatic deep vein thrombosis

作　　者：莫建文[1] 黄河[1] 张春强[1] 王兵[1] 何飞[1] 殷亮[1] 徐军[1] 陈昊[1] 赵学凌[1] 李世和[1] 

机构地区：[1]昆明医学院第一附属医院骨科,650032

出　　处：《中国急救医学》2008年第3期244-246,共3页Chinese Journal of Critical Care Medicine

基　　金：云南省自然科学基金面上项目(No2005C0071M);云南省科技厅重大攻关项目(No2005NG09)

摘　　要：目的应用基因芯片技术研究创伤性深静脉血栓(TDVT)形成过程中补体相关基因的表达变化规律,探讨其在创伤性深静脉血栓形成中的意义。方法将150只SD大鼠随机分为正常对照(A)组和模型组。模型组根据造模后的不同生物学状态再分为6组:创伤即刻(B)组、血栓形成前期(C)组、高峰期血栓形成(D)组、血栓消退期(E)组、血栓不消退(F)组和血栓不形成(G)组,在相应时相点无创切取股静脉血管组织,随后抽取总RNA,用Genechip RatGenome4302.0芯片测定股静脉RNA表达并分析补体基因表达变化情况。结果在深静脉血栓形成演化过程中,共找到与补体相关基因73个,其中已知功能基因共33个,未知基因共40个,48个基因无差异表达,包括Acdc、Adn、Bf、C1qb、C2、C3、C4bpa、C5r1、C6、Cfi、Masp1、Masp2、Pfc、S100b等在内的25个基因呈显著差异性表达。结论在创伤性肢体深静脉血栓形成中,补体相关基因是影响血栓生物学状态的重要因素之一。Objective Applying Genechip Rat Genome 430 2.0 to study the expression changes of genes related to complement in the traumatic deep vein thrombosis（TDVT） in rat models ,to explore the significance of complement in the TDVT. Method 150 rats were divided into control （ group A ） and experience groups. The experience group were divided into 6 groups according to biological states ： the post - traumatic instant （ group B ） , the initial period of thrombosis （ group C ） , the crest - time of thrombosis （ group D ） , thrombi solution （ group E ） , thrombi insolution （ group F ） and no thrombosis （ group G） groups. To incise the femoral vein in corresponding time, to adopt to Trizol one - step method for total RNA extraction of femoral vein. After applying Genechip Rat Genome 430 2.0 genechips to detect the RNAs expressions, the genes related to janus protein tyrosine kinase - signal transducer and activator of transcription signaling pathway were selected through genechip data analyasis. Results 73 genes were related to complement system. Comparing to control group, 33 genes had functional notations ,40 genes had not exactly functional notation, 45 genes had not differential expression, 25 genes of them such as Acdc, Adn, Bf, C1qb, C2, C3, C4bpa, CSrl, C6, Cfi, Maspl, Masp2, Pfc, S100b were differential expression in the process of TDVT. Conclusion In the process of TDVT, the genes related to complement were one of the most important factors to influence the biological states of thrombi.

关 键 词：创伤 深静脉血栓形成 补体系统 基因芯片 

分 类 号：R654.3[医药卫生—外科学]