超声波激活纳米氧化锌损伤牛血清白蛋白的研究  

Damage of Bovine Serum Albumin from Nano-sized Zinc Oxide under Ultrasonic Irradiation

作　　者：王君[1] 王诗献[1] 张朝红[2] 张媛媛[1] 张蕾[1] 王晓芳[3] 张向东[1] 

机构地区：[1]辽宁大学化学系,沈阳110036 [2]辽宁大学环境系,沈阳110036 [3]辽宁大学药学系,沈阳110036

出　　处：《无机化学学报》2008年第3期461-466,共6页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金资助项目(No.20371023);辽宁省教育厅自然科学基金资助项目(No.2004C018)

摘　　要：利用紫外-可见(UV-Vis)光谱和荧光光谱研究了超声波照射激活纳米氧化锌(ZnO)粒子对牛血清白蛋白(BSA)的损伤,并考查了超声波照射时间、纳米ZnO粉末加入量、溶液酸度和照射功率等因素对BSA分子损伤的影响。结果表明,对于体系温度为37.0±0.2℃和浓度为1.0×10-5mol·L^-1的BSA溶液,BSA的损伤程度随着超声波照射时间,纳米ZnO粉末加入量,溶液pH值和照射功率的增加而增大。此外,还初步探讨了超声波照射激活纳米ZnO粒子损伤BSA分子的机理,认为是声致发光和高热激发使纳米ZnO粒子产生·OH自由基,进而损伤BSA分子。The damage of bovine serum albumin （BSA） under ultrasonic irradiation in the presence of nano-sized zinc oxide （ZnO） particles was studied by UV-Vis and fluorescence spectroscopy. The influences of irradiation time, ZnO addition amount, solution acidity and irradiation power on the damage of BSA in aqueous solution were also studied. The results show that, for BSA solution of 1.0×10^-5 mol·L^-1 at 37.0±0.2 ℃, the damage degree of BSA is increased with the increase of irradiation time, ZnO addition amount, pH value and irradiation power. In addition, the possible mechanism on the damage of BSA molecule in the presence of nano-sized ZnO powders under ultrasonic irradiation was discussed. It is considered that the damage of BSA molecule is attributed to the formation of ·OH radicals resulted from the sonoluminescence and high-heat excitation.

关 键 词：超声 纳米氧化锌 损伤 牛血清白蛋白 

分 类 号：O614.241[理学—无机化学] O657.3[理学—化学]