转Bt基因抗虫棉叶片光合生产特征的探讨  

Characteristics of Photosynthetic Production in Bt Transgenic Cotton Leaves

作　　者：王永慧[1] 杨朝华[1] 张祥[1] 张丽[1] 王书红[1] 王进友[1] 陈德华[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点开放实验室,扬州225009

出　　处：《棉花学报》2008年第2期128-132,共5页Cotton Science

基　　金：江苏省教育厅重点实验室基金(K04007);农业部转基因专项基金

摘　　要：2003—2004年在江苏高肥力棉田,对9个转Bt基因棉花品种(系)的生长发育特点及其光合生产特征进行了研究。结果表明,中棉所29等3个转Bt基因杂交种在盛蕾期至盛铃期表现为叶绿素和可溶性糖含量高,净光合速率强,生殖器官干物质高,成铃强度大,蔗糖转化酶活性较高,光合生产表现为全生育期旺盛型;常规转Bt基因抗虫棉GK19和GK99088盛花前叶绿素含量、净光合速率和可溶性糖含量等光合生产特征旺盛;科棉4号和鲁棉研22表现为盛花后叶绿素含量,净光合速率,可溶性糖等光合生产特征旺盛;sGK321和鲁棉研16整个生育期叶绿素含量、叶片光合速率等光合生产特征较弱。相关分析表明,叶片蔗糖转化酶活性和生殖器官干重、成铃强度呈显著正相关,相关系数分别为0.9842**和0.8699。生产上应根据不同类型Bt棉的光合生产特征,采取不同的促控措施,以发挥光合生产潜力,提高产量。The growth and development and photosynthetic production characteristics of Bacillus thuringiensis （Bt） transgenic cotton cultivars in transplanting pattern under high fertility, during the 2003 and 2004 growing seasons at the Yangzhou University Farm, Yangzhou of Jiangsu Province, China. The results showed that Bt transgenic hybrid cotton （CCRI 29, Lumian 15 and Kemian 3） had higher contents of chlorophyll, soluble sugar, higher net photosynthetic rate, reproductive organs dry matter weight, and greater intensity for boll setting, enzyme activity of Sucroase from peak square to peak boll period. They had strong photosynthetic production in whole period. The cultivars of GK 19 and GK 99088 had higher contents of chlorophyll, soluble sugar, higher net photosynthetic rate before peak flower period; the cultivars （Kemian 4 and Lumian 22） had higher contents of chlorophyll, soluble sugar, higher net photosynthetic rate after peak flower period; the cultivars of sGK 321 and Lumian 16 had lower contents of chlorophyll, lower net photosynthetic rate in whole period. There were significant correlation between Sucroase activity and reproductive organs dry matter weight, boll-setting intensity, respectively （r=0. 9482 ^＊＊ ,r=0.8699 ^＊＊）. These results suggested that appropriate cultivating measures should be adopted concerning with different photosynthetic production characteristics of Bt cotton cultivars, which contributed to bolster the expression of photosynthetic production and improve yield.

关 键 词：BT棉 光合生产 光合特征 

分 类 号：S562[农业科学—作物学]