微量全血诱生γ-干扰素的酶免疫测定法  

An Enzyme Micro-immunoassay for Interferon (IFN)-? Induced in Whole Blood

作　　者：王艾丽[1] 杨正[1] 江舟[1] 王娟[1] 武建国[1] 

机构地区：[1]南京军区南京总医院全军医学检验中心,南京210002

出　　处：《上海免疫学杂志》1997年第1期47-49,共3页Shanghai Journal of Immunology

摘　　要：建立了一种微量全血诱生γ-干扰素(IFN-γ)的酶免疫测定法.将抗凝全血、脂多糖(LPS)、植物血凝素(PHA)、RPMI 1640培养基和HRP标记的抗IFN-γ单克隆抗体,同置于用抗IFN-γ多克隆抗体包被的反应板微孔中.此法IFN-γ最小检出量为0.5ng/ml,特异性、重复性均较好,适于临床应用.An enzyme micro-immunoassay for determination of IFN-γ induced in who-le blood (WB) was developed. Microtiter plates were coated with specific polyclonal antibody against IFN-γ. WB(25μ1), polyclonal activitors (lipopolysaccharide and phy-tohemagglutinin, LPS/PHA) and RPMI 1640 medium containing specific peroxidase labelled monoclonal antibody against IFN-γ were incubated together in the wells of plates. IFN-γ induced in kinetics was captured. The Efficiency of the assay reached a lower detecting limit up to 0.5 ng/ml. The results showed a fine agreement with tho-se obtained by a two-step routine ELISA which was performed following separate step of inducing IFN-? (r = 0.856). This specific and reliable assay is easy to set up in clinical laboratories.

关 键 词：全血 Γ-干扰素 ELISA 细胞免疫功能 

分 类 号：R446.63[医药卫生—诊断学]