RGD对人纤维肉瘤细胞HT1080增殖、黏附及转移影响的研究  

Studies on the effects of RGD peptide on the proliferation,adherence and motility of human fibrosarcoma cell HT1080

作　　者：侯瑞珍[1] 王巍[2] 李春蓉[1] 徐力[1] 张学忠[1] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,吉林长春130023 [2]长春工业大学化工学院,吉林长春130012

出　　处：《东北师大学报（自然科学版）》2008年第1期120-124,共5页Journal of Northeast Normal University(Natural Science Edition)

基　　金：教育部留学回国人员科研启动基金资助项目;吉林省科技发展计划项目(20070926-03)

摘　　要：利用合成的RGD(Arg-Gly-Asp)三肽研究了其抑制人纤维肉瘤细胞HT1080的增殖、黏附及转移作用.采用MTT法检测了不同浓度的RGD对HT1080细胞增殖及黏附纤维粘连蛋白(fibronectin)能力的影响;采用细胞划痕法研究了RGD对HT1080细胞在纤维粘连蛋白中迁移能力的影响.结果表明,合成的RGD能抑制HT1080细胞的增殖,并具有抗肿瘤细胞黏附、抗转移的活性.The effects of the synthetic tripeptide RGD on the proliferation, adherence andmotility of human fibrosarcoma cell HT1080 have been studied. MTT assay was applied to study the effects of RGD peptide on HT1080 cell proliferation and adhesive capability to Fibronectin. Cellular motility experiment was carried out to assay the inhibitory effect of RGD peptide on HT1080 motility on Fibronectin. The results indicated that RGD peptide had inhibitory effects on the proliferation and adherence of HT1080 cells to fibronectin. It also could restrain motility of HT1080 cells on fibronectin.

关 键 词：RGD 增殖 黏附 肿瘤转移 

分 类 号：Q74[生物学—分子生物学]