内参比pH纳米传感器用于不同抗肿瘤药物作用的Hela细胞内pH变化的测量  

Intracellular pH detection of different anti-tumor drugs treated Hela cells by using ratiometric pH nanosensors

作　　者：王燕[1] 何晓晓[1] 王柯敏[1] 谭蔚泓[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室,湖南大学生物医学工程中心,湖南大学生命科学与技术研究院,湖南长沙410082

出　　处：《化学传感器》2008年第1期19-25,共7页Chemical Sensors

基　　金：国家重点基础研究发展规划(2002CB513100-10);国家自然科学基金重大研究计划重点项目(90606003);国家自然科学基金项目(20405005);教育部重点项目(107084);湖南省杰出青年基金资助(06JJ10004)

摘　　要：采用该研究小组发展的pH敏感染料异硫氰酸荧光素(FITC)和参比染料联钌吡啶(RuBpy)同时包埋的具有内参比功能的pH纳米传感器,进一步结合流式细胞学分析方法,对不同抗肿瘤药物作用的Hela细胞内的pH变化进行了分析,结果表明:不同抗肿瘤药物作用后的Hela细胞内pH的变化情况并不一样,有的作用后引起了细胞酸化,有的作用后引起了细胞碱化,有的作用后基本上不引起变化。同时,采用激光共聚焦显微镜对吞噬了内参比pH纳米传感器的抗肿瘤药物作用的单个Hela活细胞进行了成像,可以明显观察到内参比pH纳米传感器在不同抗肿瘤药物作用后的Hela细胞内的荧光成像。The intracellular pH of different anti-tumor drugs treated Hela cells had been detected by combination with the advantages of the small size of the ratiometric pH nanosensors that have been developed by our group and the flow cytometry analysis method. The results demonstrated that the intracellular pH change was different when the Hela cells was treated with different anti-tumor drugs, some would caused intracellular acidification, some would caused intracellular basification, and some would barely caused pH change. The single living Hela cells uptaken with the ratiometric pH nanosensor was also imaged with laser confocal microscope, which was treated with anti-tumor drugs.

关 键 词：内参比pH纳米传感器 抗肿瘤药物 细胞内pH 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]