HIV 外膜糖蛋白 gp120 和 gp41 在昆虫细胞中表达及其免疫学特性的评价  

Expression of HIV env protein in insect cells and evaluation of their immunological properties

作　　者：董明[1] 谢云[1] 项金忠[1] 尚继军[1] 张鹏飞[1] 

机构地区：[1]卫生部北京生物制品研究所

出　　处：《中华微生物学和免疫学杂志》1997年第1期7-11,共5页Chinese Journal of Microbiology and Immunology

摘　　要：将编码完整ｇｐ１２０和完整ｇｐ４１的基因分别克隆到杆状病毒转移质粒中。使用重组转移质粒与野生杆状病毒（ＡｃＮＰＶ）ＤＮＡ共转染Ｓｆ９昆虫细胞，经挑选获得分别带有编码ｇｐ１２０和ｇｐ４１基因的重组杆状病毒。重组杆状病毒感染Ｓｆ９细胞后在细胞中分别表达了ＨＩＶ外膜糖蛋白ｇｐ１２０和ｇｐ４１。其重组蛋白的分子量分别为１２０×１０３和４１×１０３。此重组糖蛋白在免疫荧光、免疫印染和酶联免疫实验中都能被ＨＩＶ阳性血清所识别。动物免疫实验表明此重组糖蛋白能诱导很强的特异性抗体产生。The full length cDNA coding HIV gp120 and gp41 env proteins were cloned from pZHIV plasmid containing HIV genome and insterted into baculovirus transfer vector.Sf9 insect cells were co transfected with the recombinant transfer vector plasmids and wild type baculovirus DNA.The recombinant baculoviruses containing genes of HIV gp120 and gp41 were obtained by limited dilutions.120kD and 41kD recombinant HIV env proteins were expressed by Sf9 cells infected with recombinant viruses.The recombinant proteins could be recognized by human HIV positive sera evidenced by Western blot and EIA,and also could elicit specific anti HIV antibodies in animals.

关 键 词：昆虫细胞 糖蛋白 包膜蛋白质 艾滋病病毒 免疫性 

分 类 号：R373.9[医药卫生—病原生物学] R392.11[医药卫生—基础医学]