微量稀释法测定皮肤癣菌最低抑菌浓度(MIC)的实验研究  

Study on determining MIC of itraconazole and fluconazole against dermatophyte by microdilution test

作　　者：刘维达[1,2,3] 王金燕[1,2,3] 吴建明 

机构地区：[1]中国医学科学院中国协和医科大学皮肤病研究所 [2]内蒙古医学院附院皮肤科 [3]江苏省建湖县皮防所

出　　处：《临床皮肤科杂志》1997年第4期228-230,共3页Journal of Clinical Dermatology

摘　　要：目的，建立用微量稀释法测定皮肤癣菌最低抑菌浓度（ＭＩＣ）的方法学，为该类实验的标准化打下基础。方法：用９６孔酶标板做药敏试验载体，１０级倍比稀释药液，测定３０株皮肤癣菌临床分离株对三唑类新药伊曲康唑和氟康唑的敏感性。结果：３０株皮肤癣菌对伊曲康唑和氟康唑的ＭＩＣ范围分别为≤０．０６～４μｇ／ｍｌ和１～３２μｇ／ｍｌ，均值分别为０．３２μｇ／ｍｌ和７．７６μｇ／ｍｌ．结论：用微量稀释法测定皮肤癣菌ＭＩＣ初步显示了用药量少、重复性好、结果较可靠等优点。Objective: to set up a test methodology of determining MIC of dermatophytes by using a microdilution test, and provide a basis for the standardized tests in future. Methods: using 96 well microtiter trays as testing carriers, and ten twofold serial dilutions were made. The susceptibilities of 30 dermatophyte strains isolated from clinic to itraconazole and fluconazole, two new azole antifungal drugs, were determined. Results: the MIC range of itraconazole and fluconazole against 30 dermatophyte strains are 0.06～4μg/ml and 1～32μg/ml, respectively, with mean being 0.32μg/ml and 7.76μg/ml,respectively. Conclusion: the advantages of dermining dermatophyte's MIC to itraconazole and fluconazole by microdilution technique are economy of the test drugs and satifactory reproduction and neliability.

关 键 词：皮肤癣菌 伊曲康唑 最低抑菌浓度 微量稀释法 

分 类 号：R756.05[医药卫生—皮肤病学与性病学] R978.5[医药卫生—临床医学]