Effects　of　advanced　glycosylation　end　products　on　proliferation　and　cytosolic　free　calcium　in　cultured　rat　aortic　smooth　muscle　cells  

作　　者：金晖[1] 刘乃丰[1] 唐尧[1] 

机构地区：[1]南京铁道医学院附属第一医院心内科

出　　处：《中国药理学报》1997年第5期422-425,共4页Acta Pharmacologica Sinica

摘　　要：目的：研究糖基化终产物（ＡＧＥＰ）对主动脉平滑肌细胞增殖的影响及其与［Ｃａ２＋］ｉ的关系．方法：采用同位素掺入法分别测定ＤＮＡ和蛋白质合成；Ｆｕｒａ２ＡＭ测定［Ｃａ２＋］ｉ．结果：ＡＧＥＰ以浓度、时间相关的方式促进［３Ｈ］ＴｄＲ与［３Ｈ］Ｌｅｕ掺入细胞，随ＡＧＥＰ作用时间、糖化时间延长，掺入率增加明显．ＡＧＥＰ增加［Ｃａ２＋］ｉ，与时间、浓度相关，但随ＡＧＥＰ作用时间延长（４０分钟后）而有所降低，ＢＳＡ修饰中葡萄糖浓度的增加，糖基化时间延长，［Ｃａ２＋］ｉ也呈上升趋势．结论：ＡＧＥＰ刺激平滑肌细胞增殖，并与细胞［Ｃａ２＋］ｉ浓度增加有关．AIM: To study the effects of advanced glycosylation end products (AGEP) on aortic smooth muscle cell (ASMC) proliferation and its relationship with cytosolic free calcium ([Ca 2+ ] i). METHODS: The effects of AGEP modified bovine serum albumin (AGEP BSA) on the incorporation of TdR and Leu into cultured ASMC were observed. The [Ca 2+ ] i of cultured ASMC was determined with Fura 2 AM . RESULTS: AGEP BSA stimulated the incorporation of TdR and Leu into ASMC in concentration and time dependent manners ( P <0 01), especially TdR. In AGEP 200 mg·L -1 group, after 12 h exposure, the incorporation of TdR obviously increased, but DNA synthesis was concentration dependently decreased in AGEP 300-400 mg·L -1 groups. The peak incorporation of TdR was 10 times vs control ( P <0 01). The [Ca 2+ ] i of ASMC incubated with AGEP BSA for 40 min was increased from control groups 121±4 to 492±20 nmol·L -1 ( P < 0 01). [Ca 2+ ] i induced by AGEP was elevated with the concentration and incubating time of glucose with BSA. [Ca 2+ ] i in BSA incubated with glucose 80 mmol·L -1 for 12 wk was 580±37 nmol·L -1 ( P <0 01). CONCLU￣SION: AGEP stimulated proliferation of ASMC associated with the elevation of [Ca 2+ ] i.

关 键 词：糖基化终产物 钙 血管平滑肌 糖尿病 血管病变 

分 类 号：R587.2[医药卫生—内分泌] R543.5[医药卫生—内科学]