Effect　of　losartan　and　captopril　on　expression　of　cardiac　angiotensin　Ⅱ　AT1　receptor　mRNA　in　rats　following　myocardial　infarction  

作　　者：张高星[1] 浦寿月[1] 杨英珍[1] 沈学东[1] 彭天庆[1] 陈灏珠[1] 

机构地区：[1]上海医科大学附属中山医院上海市心血管病研究所卫生部病毒性心脏病重点实验室

出　　处：《中国药理学报》1997年第5期431-434,共4页Acta Pharmacologica Sinica

摘　　要：目的：探讨氯沙坦（Ｌｏｓ）和卡托普利（Ｃａｐ）对大鼠心肌梗死后血管紧张素Ⅱ（Ａｎｇ）ＡＴ１受体ｍＲＮＡ表达的影响．方法：２４只梗死大鼠随机分为四组并分别用Ｃａｐ（２ｇ·Ｌ－１加水中饮用）、Ｌｏｓ（１０ｍｇ·ｋｇ－１·ｄ－１和３０ｍｇ·ｋｇ－１·ｄ－１，灌胃）及安慰剂治疗６周，假扎鼠作对照，用地高辛标记的ｃＤＮＡ探针，进行点杂交反应，检测ＡｎｇＡＴ１受体ｍＲＮＡ表达．结果：三个药物治疗组的ＡｎｇＡＴ１受体ｍＲＮＡ表达水平分别与安慰剂组比较，均显著降低（Ｐ＜００１）．三个治疗组之间的ＡｎｇＡＴ１受体ｍＲＮＡ表达水平及分别与假扎组比较，均无显著差异（Ｐ＞００５）．结论：Ｃａｐ和Ｌｏｓ均可逆转大鼠心肌梗死后ＡｎｇＡＴ１受体ｍＲＮＡ表达水平的增高．AIM: To study the effects of losartan (Los) and captopril (Cap) treatment on expression of cardiac angiotensin Ⅱ (Ang) AT 1 receptor mRNA in rats following myocardial infarction (MI). METHODS: Twenty four rats with MI after coronary ligation for 7 d were randomly divided into 4 groups: A) Cap in drinking water, ad lib (2 g·L -1 ), B) ig Los 10 mg·kg -1 ·d -1 , C) ig Los 30 mg·kg -1 ·d -1 , and D) placebo for 6 wk. Sham ligation rats (group E) served as controls. The levels of cardiac Ang AT 1 receptor mRNA expression in each group ( n =6) were examined by Dot blot using digoxigenin labeled cDNA probes. RESULTS: Comparing with reflected peak areas of hybridization positive signals in group D (2640±201 μm 2) , the expression of the cardiac Ang AT 1 receptor mRNA was much lower in the 3 treated groups (group A 1360±134 μm 2, group B 1430±244 μm 2, group C 1310±95 μm 2) ( P <0 01). But no difference was found between the 3 treated groups and sham ligation group (1230±233 μm 2) ( P >0 05). CONCLUSION: Los and Cap attenuated the increase of cardiac Ang AT 1 receptor mRNA expression in rats following MI.

关 键 词：MRNA 卡托普利 氯沙坦 

分 类 号：R972.4[医药卫生—药品] R542.220.5[医药卫生—药学]