Detection　of　DNA　damage　in　peripheral　lymphocytes　by　7　compounds　using　comet　assay  

作　　者：王吉成[1] 钱蓓丽[1] 

机构地区：[1]上海医药工业研究院

出　　处：《中国药理学报》1997年第5期451-454,共4页Acta Pharmacologica Sinica

摘　　要：目的：检测过氧化氢（Ｈ２Ｏ２）、甲磺酸乙酯（ＥＭＳ）、丝裂霉素Ｃ（ＭＭＣ）、二甲基亚硝胺（ＤＭＮＡ）、苯并（ａ）芘（ＢａＰ）、２氨基芴（２ＡＦ）和环磷酰胺（ＣＰ）诱发小鼠、大鼠及人外周血淋巴细胞ＤＮＡ单链断裂．方法：体外单细胞微量凝胶碱性电泳试验（慧星试验）．结果：除ＥＭＳ０９７ｍｍｏｌ·Ｌ－１在小鼠淋巴细胞，ＭＭＣ３０μｍｏｌ·Ｌ－１在小鼠、人淋巴细胞中呈阴性外，其余均为阳性．最低可检测浓度分别为Ｈ２Ｏ２１μｍｏｌ·Ｌ－１，ＥＭＳ０４８ｍｍｏｌ·Ｌ－１，ＢａＰ５０μｍｏｌ·Ｌ－１，ＣＰ２０ｍｍｏｌ·Ｌ－１，ＭＭＣ１０μｍｏｌ·Ｌ－１，ＤＭＮＡ２７３ｍｍｏｌ·Ｌ－１，２ＡＦ６２５μｍｏｌ·Ｌ－１．ＣＰ、ＢａＰ、２ＡＦ需经Ｓ９Ｍｉｘ代谢活化才显示毒性．结论：彗星试验检测出ＭＭＣ诱导大鼠，ＥＭＳ诱导大鼠和人，以及Ｈ２Ｏ２、ＤＭＮＡ、ＢａＰ、ＣＰ和２ＡＦ诱导小鼠、大鼠和人外周血淋巴细胞ＤＮＡ单链断裂损伤．AIM: To detect the DNA single strand breaks (SSB) in peripheral lymphocytes of mice, rats, and human induced by hydrogen peroxide (H 2O 2), ethyl methane sulphonate (EMS), dimethylnitrosamine (DMNA), mitomycin C (MMC), benzo(a)pyrene (BaP), cyclophosphamide (CP), and 2 aminofluorene (2 AF) . METHODS: Alkaline single cell microgel electrophoresis assay in vitro (comet assay). RESULTS: All were positive with 2 exceptions: EMS (0 97 mmol·L -1 ) in mice and MMC (30 μmol·L -1 ) in mice and human. The lowest concentrations detectable were H 2O 2 (1 μmol·L -1 ), EMS (0.48 mmol·L -1 ), BaP (5 0 μmol·L -1 ), CP (2 0 mmol·L -1 ), MMC (10 μmol·L -1 ), DMNA (27 3 mmol·L -1 ), and 2 AF (62 5 μmol·L -1 ). CP, BaP, and 2 AF were positive only in the presence of metabolic activation system. CONCLUSION: H 2O 2, DMNA, BaP, CP, and 2 AF induce SSB in peripheral lymphocytes of mice, rats, and human detected by comet assay, whereas MMC induces SSB only in rats, and EMS in rats and human lymphocytes.

关 键 词：凝胶电泳 甲磺酸乙酯 淋巴细胞 DNA损伤 

分 类 号：R992[医药卫生—毒理学]