鼻窦炎鼻息肉组织及分泌物中嗜酸粒细胞主要碱性蛋白和中性粒细胞弹性蛋白酶的表达  

Expression of eosinophil major basic protein and neutrophil elastase in nasal polyp tissue and secretion

作　　者：张淳[1] 江一鸣[1] 陈婕[1] 郑潞滢[2] 王家东[1] 沈惠风[3] 

机构地区：[1]上海交通大学医学院附属仁济医院耳鼻咽喉-头颈外科,上海200001 [2]上海交通大学医学院附属仁济医院病理科 [3]上海交通大学医学院附属仁济医院中医科

出　　处：《临床耳鼻咽喉头颈外科杂志》2008年第4期166-168,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:通过比较嗜酸粒细胞主要碱性蛋白(MBP)和中性粒细胞弹性蛋白酶(NE)在鼻息肉(NP)黏膜上皮、固有层及分泌物中的表达,探讨它们在NP发病机制中的作用。方法:采用免疫组织化学和Axioplan 2显微图像分析系统检测30例慢性鼻窦炎鼻息肉患者的NP上皮、固有层及分泌物中MBP和NE的表达情况。结果:①MBP和NE在上皮组织和分泌物中的表达与对照组相比均差异有统计学意义(均P<0.05);②MBP和NE在上皮组织和固有层内的平均阳性表达均差异无统计学意义(均P>0.05),而在固有层内的表达差异有统计学意义(P<0.05);③MBP的平均阳性表达在上皮、固有层和分泌物中差异有统计学意义(P<0.05);④NE的平均阳性表达在上皮、固有层和分泌物中差异有统计学意义(P<0.05);⑤分泌物中MBP、NE以脱颗粒状态存在为主,而在上皮和固有层内分别以存在于完整的嗜酸粒细胞和中性粒细胞内为主;⑥上皮脱落区域均有大量MBP、NE表达,而上皮完整区域及固有层内仅有少量MBP、NE表达;⑦电镜分别显示MBP和NE颗粒电子密度特征。结论:MBP和NE在NP的发生中共同发挥病理学作用。Objective：To explore the role in the pathogenesis of nasal polyp （NP） by comparison of eosinophil major basic protein （MBP）, and neutrophil elastase （NE） in nasal polyps （NP） epithelium, stroma and the secretion of expression. Method：Immunohistochemical detection of 30 cases of patients with chronic sinusitis （CRS） NP epithelium NE stroma and the expression and secretion of MBP. Result：①There were significant differences of the expression of NE and MBP in epithelial tissue, stroma and secretion compared with the control group （P〈0.05） ; ②There was not significant difference of the expression of NE and MBP between epithelial tissue and stroma （P〉0. 05）, while there was significant difference between epithelial tissue and the secretion （P〈0.05） ;③There were significant differences of the average positive expression of MBP and NE among epithelial tissue, stroma and secretion （P〈0.05） ;④MBP and NE were usually degranulated in secretion, while usually located in eosinophils （Eos） and neutrophils （Neu） in epithelial and mesenchymal;⑤There were abundant expression of MBP and NE in epithelial shedding regional, while small amounts of expression in stroma and integrited epithelial;⑥Electron microscopy could show the characteristics of electron density of MBP and NE particles. Conclusion： MBP and NE collaborated to cause pathological effects on the occurrence of NP.

关 键 词：鼻窦炎 鼻息肉 嗜酸粒细胞碱性蛋白 中性粒细胞弹性蛋白酶 

分 类 号：R765.41[医药卫生—耳鼻咽喉科]