氧化型染发剂DNA交联作用的研究  

Study on Interstrand DNA Cross links in Calf Thymus DNA Caused by Oxidized Hairdyes

作　　者：杨薇[1] 戴修道[1] 浦跃朴[2] 尹立红[2] 

机构地区：[1]上海医科大学公共卫生学院 [2]南京铁道医学院预防医学系

出　　处：《中国公共卫生学报》1997年第5期291-292,共2页

摘　　要：采用溴乙锭荧光法测定氧化型染发剂的主要成分对苯二胺（ＰＰＤ）、过氧化氢（Ｈ２Ｏ２）和ＰＰＤ－Ｈ２Ｏ２混合物对小牛胸腺ＤＮＡ的双链间交联形成作用。结果表明：用０～６％的对苯二胺及０～３％的Ｈ２Ｏ２对小牛胸腺ＤＮＡ直接染毒，３７℃３０ｍｉｎ时无双链间ＤＮＡ交链的形成；而ＰＰＤ和Ｈ２Ｏ２发生氧化反应后则具有双链ＤＮＡ交联形成作用并具有剂量反应关系（ｒ＝０．８２９，Ｐ＜０．０１），在受试剂量为０～０．８％（以对苯二胺含量计）时，氧化型对苯二胺的ＤＮＡ交联作用增加了４．２倍。研究结果提示，ＤＮＡ交联作用可能是氧化型染发剂遗传毒作用过程中ＤＮＡ损伤的一种方式。Genotoxicity of oxidized hairdyes were introduced by a few of studies.The interstrand DNA cross links of calf thymus DNA by oxidized hairdyes and their main gradients:phenylenediamine and hydrogen peroxide were measured with ethidium bromide assay in this study.It was indicated that PPD and H 2O 2 had not DNA cross links on calf thymus DNA;oxidized PPD had the dose responsive effect of DNA cross links on calf thymus DNA (r=0.829,P<0.01).It was suggested that DNA cross links is one way of NDA damage in the process of the genotoxicity of oxydized hairdyes.

关 键 词：氧化型 染发剂 对苯二胺 DNA交联 遗传毒性 

分 类 号：R996[医药卫生—毒理学] R992[医药卫生—药学]