单细胞电泳检测冰冻保存对外周血细胞DNA的损伤  

DNA Damage Caused by Cryopreservation of Peripheral Blood Cells Detected by Single Cell GE 1 Assay

作　　者：倪祖尧[1,2,3,4,5] 逢兵[1,2,3,4,5] 孟建锋 杨成峰 刘玉清[1,2,3,4,5] 李寿祺[1,2,3,4,5] 蒋学芝 庄志雄[1,2,3,4,5] 张桥 

机构地区：[1]华西医科大学卫生毒理教研室 [2]上海医科大学劳动卫生学教研室 [3]北京医科大学预防医学教研室 [4]同济医科大学环境卫生教研室 [5]中山医科大学劳动卫生教研室

出　　处：《中国公共卫生学报》1997年第5期313-315,共3页

基　　金：CMB资助

摘　　要：用对ＤＮＡ损伤十分敏感的单细胞电泳技术测试冰冻对正常人外周血细胞ＤＮＡ的损伤效应。结果显示－２０℃和－８０℃冰冻后慧星细胞百分率比４℃保存样品明显增多，损伤程度亦加剧。相对而言４℃保存ＤＮＡ较稳定，但随时间的延长ＤＮＡ完整性受到破坏也越严重。DNA damage is of concern to research in many fields,especially cancer research and toxicology.To evaluate the possible loss of DNA structural integrity during freezing of cells collected over a periol of time,recently developed single cell gel(SCG) assay has been employed to measure the effects of DNA damage in individual peripheral blood cells stored at 4℃,-20℃and -80℃,respectively.The results showed that the basal ratio of comet cells in frozen cells was significantly higher than that of fresh cells.The increase of the frozen time can enhance the effects of DNA damage.The results suggested that evaluation of DNA damage of cells stored by cryopreservation may produce artificially exaggerated levels of damage,which could limit analytical interpretation.

关 键 词：外周血细胞 单细胞电泳 DNA损伤 冰冻 毒理学 

分 类 号：R991[医药卫生—毒理学]