培养基成分对怀牛膝愈伤组织诱导及牛膝多糖含量的影响  

Effect of medium components on callus induction and contents of polysaccharides in calli from Achyranthes bidentata

作　　者：李明军[1] 李萍[1] 张晓丽[1] 赵喜亭[1] 

机构地区：[1]河南师范大学生命科学学院

出　　处：《中国中药杂志》2008年第7期745-749,共5页China Journal of Chinese Materia Medica

基　　金：河南省重点科技攻关项目(0623030700,0524420001)

摘　　要：目的:研究培养基成分对怀牛膝愈伤组织诱导形成及牛膝多糖含量的影响。方法:以怀牛膝无菌苗的叶片、茎段为材料,采用正交设计研究基本培养基,碳源,2,4-二氯苯氧乙酸(2,4-D)、6-苄基腺嘌呤(6-BA)、N-苯基-N′-噻二唑-5-脲(TDZ)、水解酪蛋白(CH)对愈伤组织诱导和多糖含量的影响。结果:诱导叶片愈伤组织形成的适宜培养基为B5+2mg·L-12,4-D+0.5mg·L-16-BA+30g·L-1葡萄糖+1g·L-1CH,诱导茎段愈伤组织形成的适宜培养基为B5+2mg·L-12,4-D+1mg·L-16-BA+30g·L-1葡萄糖+0.5g·L-1CH;有利于叶片愈伤组织中多糖含量提高的培养基是LS+1mg·L-16-BA+1mg·L-1TDZ+30g·L-1蔗糖,有利于茎段愈伤组织中多糖含量提高的培养基是LS+1mg.L-12,4-D+0.5mg·L-16-BA+1mg·L-1TDZ+30g·L-1葡萄糖。结论:初步建立了怀牛膝叶片、茎段诱导愈伤组织的适宜培养条件,为通过植物组织细胞培养途径生产牛膝多糖提供了依据。Objective： To study the effect of medium components on the callus induction and the contents of polysaccharides in calli from Achyranthes bidentata. Method： Leaves and stems were selected as explants. The effects of six kinds of factors including basal culture medium, carbon source, 2,4-D, 6-BA, TDZ, CH on the callus induction and the contents of ABPS in calli on the high growth point were studied by orthogonal design method. The data were analyzed with range analysis and variance analysis. Result： To leaf, the optimal medium of callus induction was B5 with 2 mg · L^-1 2,4-D, 0.5 mg · L^-1 6-BA , 30 g · L^-1 glucose and 1 g · L^-1 CH; to stem, the optimal one was B5 with 2 mg·L^-1 2,4-D, 1 mg · L^-1 6-BA, 30 g · L^-1 glucose and 0.5 g· L^-1 CH. In order to obtain higher contents ABPS, to leaf calli, the optimal medium was LS with 1 mg · L^-1 6-BA, 1 mg· L^-1 TDZ and 30 g · L^-1 su- crose; to stem calli, the optimal one was LS with 1 mg · L^-1 2,4-D, 0.5 mg·L^-1 6-BA, 1 mg · L^-1 TDZ and 30 g· L^-1 glucose. Conclusion： The optimal media of callus induction were established with stems and leaves of A. bidentata as explants and with a view to an industrial production of polysaccharides by tissue and cell culture.

关 键 词：怀牛膝 愈伤组织 多糖 培养基成分 正交设计 

分 类 号：R284.1[医药卫生—中药学] S567[医药卫生—中医学]