大黄素对大鼠肝移植后肝细胞凋亡的影响  

Effect of emodin on hepatocellular apoptosis following orthotopic liver transplantation in rats

作　　者：林胜璋[1] 徐贤绸[1] 荆河[1] 杨潇[1] 

机构地区：[1]温州医学院附属第二医院外科,浙江温州325003

出　　处：《中国中药杂志》2008年第7期809-812,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30572395)

摘　　要：目的:探讨大黄素对大鼠肝移植后肝细胞凋亡的影响。方法:建立LEW→BN大鼠肝移植动物模型,随机分为A,B,C,D4组,每组6只动物,术后每天分别腹腔注射生理盐水0.5mL.d-1、环孢素A(CsA)10.0mg.kg-1.d-1、大黄素50.0mg.kg-1.d-1、大黄素50.0mg.kg-1.d-1+CsA10.0mg.kg-1.d-1,连续用药8d,停药7d后处死动物取肝脏组织观察排斥活动指数(RAI)和肝细胞凋亡指数(AI)。结果:A,B,C,D组RAI分别是(7.67±0.98),(5.17±0.40),(5.83±0.75),(3.83±0.75),AI分别是(35.83±2.32),(15.83±1.33),(16.50±2.35),(11.50±1.05)。B,C,D组的RAI,AI较A组均明显降低(P<0.01),而D组较B,C组也均有明显降低(P<0.05),B,C组间差别均无意义。结论:大黄素能有效减少大鼠移植肝术后肝细胞凋亡,抑制排斥反应,且和CsA有协调作用。Objective： To evaluate the effect of emodin on hepatocellular apoptosis following orthotopic liver transplantation （OLT） in rats. Method： The LEW→BN OLT models were established. A total of 24 rots were divided randomly and equally into 4 groups. Group A was treated with normal saline at dose of 0.5 mL· d^-1 intraperitoneaUy from 1st day to 8 th day after operation. Group B, CsA at dose of 10.0 mg· kg^-1 · d^-1. Group C, emodin at dose of 50.0 mg· kg^-1 · d^-1. Group D, CsA at dose of 10.0 mg· kg^-1 · d^-1 and emodin at dose of 50. 0 mg· kg^-1 · d^-1. Fifteen days after operation, rejection active index （RAI） and hepatocellular apoptosis index （AI） was confirmed after observing the pathologic change of transplanted liver in recipients. Result： Respectively, the RAI of group A, B, C, D was 7.67±0.98, 5.17±0.40, 5.83±0.75, 3.83 ±0.75 and the AI of group A, B, C, D was 35.83 ±2.320, 15.83 ±1.33, 16.50 ±2.35, 11.50 ± 1. 05. The RAI and AI of group B, C, D was significantly lower than group A （P 〈0. 01 ） and group D was significantly lower than group B, C too （P〈0.05）. There was no significant distinction between group B and C in RAI and AI. Conclusion： Emodin has the effect of reduce the hepatoccllular apeptosis following OLT in rats and the effect can stronger by CsA.

关 键 词：大黄素 肝移植 移植物排斥 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]