低分子肝素和尿激酶对大鼠肾小球炎症保护作用的比较  

Protection of low-molecular heparin and urokinase on LPS-induced glomerular inflammation in rats

作　　者：林淑芃[1] 孙雪峰[1] 陈香美[1] 师锁柱[1] 洪权[1] 吕杨[1] 

机构地区：[1]解放军总医院肾脏病研究所暨重点实验室 解放军总医院研究生队,北京100853

出　　处：《解放军医学杂志》2008年第4期382-384,共3页Medical Journal of Chinese People's Liberation Army

基　　金：“973”国家重大基础研究发展规划基金资助项目(2007CB507400);国家自然科学基金创新研究群体基金资助项目(30121005);国家自然科学基金资助项目(30470803)

摘　　要：目的比较低分子量肝素和尿激酶对肾小球炎症反应的保护作用。方法3月龄雌性Wistar大鼠40只,随机分为对照组(NC组)、脂多糖(LPS)组(L组)、LPS+氨甲环酸组(LT组)、LPS+氨甲环酸+低分子量肝素组(LTH组)及LPS+氨甲环酸+尿激酶组(LTU组),每组8只。采用直接免疫荧光检测肾小球纤维蛋白沉积和CD11b阳性细胞分布情况,Western blotting检测内皮细胞细胞间黏附分子-1(ICAM-1)蛋白质表达。结果NC组大鼠肾小球内无纤维蛋白沉积和CD11b阳性细胞;ICAM-1有少量表达。与NC组比较,L组纤维蛋白沉积(9.1%±1.6%)和CD11b阳性细胞(11.2±2.1)增多(P<0.05);ICAM-1表达(0.23±0.09)明显上调(P<0.05)。与L组比较,LT组纤维蛋白沉积(23.4%±3.2%)和CD11b阳性细胞(20.4±3.5)进一步增多;ICAM-1表达(0.44±0.16)进一步上调(P<0.05)。与LT组比较,LTH组和LTU组纤维蛋白沉积(分别为11.3%±1.4%,10.6%±1.5%)和CD11b阳性细胞(分别为7.3±1.8,8.4±1.6)明显减少(P<0.05或0.01);ICAM-1表达(分别为0.19±0.10,0.18±0.09)明显下调(P<0.05),LTH、LTU两组间无统计学差异。结论低分子肝素与尿激酶均能有效减少纤维蛋白沉积,起到减轻肾小球炎症反应的作用。Objective To investigate the protective effects of low-molecular heparin and urokinase on glomerular inflammation. Methods Forty 3-month-old female Wistar rats were randomly divided into five groups with 8 rats in each group： normal control （NC） group; lipopolysaccharide （LPS） group; LPS and tranexamic acid （LT） group; LPS, tranexamic acid and low-molecular heparin （LTH） group; and LPS, tranexamic acid and urokinase （LTU） group. Fibrin deposition and CD11b positive cells were identified by immunohistochemical staining. Western blotting was used to determine the ICAM-1 expression. Results No fibrin depositions and CD11b positive cells were found in glomeruli of rats in NC group. Compared with NC group, fibrin deposit （9.1%±1.6%）, CD11b positive cell （11.2±2.1） and ICAM-1 expressions （0.23±0.09） were significantly increased in L group （P〈0.05）. Compared with L group, fibrin deposition （11.3%±1.4%, 10.6%±1.5%, respectively）, CD11b positive cells （7.3±1.8, 8.4±1.6, respectively） and ICAM-1 expressions （0.19±0.10, 0.18±0.09, respectively） were significant lower in LTH and LTU groups （P〈0.05）. No differences of fibrin deposition, CD11b positive cells and ICAM-1 expressions were found between LTH and LTU groups （P〉0.05）. Conclusion Both low-molecular heparin and urokinase can effectively decrease fibrin deposits and alleviate inflammation.

关 键 词：肾小球肾炎 肝素 尿纤溶酶原激活物 

分 类 号：R692.31[医药卫生—泌尿科学]