NMDA、甘氨酸、阿片受体与硫喷妥钠催眠作用的关系  

Relationship between the hypnotic effect of thiopental sodium with N-methyl-D-aspartate (NMDA),glycine and opioid receptors

作　　者：李惠萍[1] 吴钢[1] 裴世成[1] 戴体俊[2] 

机构地区：[1]柳州柳州医学高等专科学校药理教研室,广西545006 [2]徐州医学院麻醉药理学教研室

出　　处：《解放军医学杂志》2008年第4期419-420,共2页Medical Journal of Chinese People's Liberation Army

基　　金：广西医药卫生计划课题项目(Z2005199)

摘　　要：目的探讨硫喷妥钠催眠作用与N-甲基-D-天冬氨酸(NMDA)受体、阿片受体和甘氨酸受体的关系。方法120只昆明种小鼠,雌雄不拘,随机分成NMDA组、纳络酮组和士的宁组。实验小鼠均于腹腔注射50mg/kg硫喷妥钠,待翻正反射消失1min后,NMDA组40只小鼠鞘内分别注射人工脑脊液(aCSF)和25、50、75ngNMDA(n=10);纳络酮组40只小鼠腹腔分别注射生理盐水(NS)和1.0、2.0、4.0mg/kg纳络酮(n=10);士的宁组40只小鼠腹腔分别注射NS和0.5、1.0、2.0mg/kg士的宁(n=10),记录小鼠睡眠时间。结果NMDA对硫喷妥钠催眠小鼠的睡眠时间无明显影响(P>0.05);而纳络酮或士的宁均能明显延长其睡眠时间(P<0.01)。结论NMDA受体与硫喷妥钠的催眠作用无明显关系,而甘氨酸受体和阿片受体均介导了硫喷妥钠的催眠作用。Objective To explore the relationship between the hypnotic effect of thiopental sodium and N-methyl-D-aspartate （NMDA） receptor, opioid receptor and glycine receptor. Methods 120 Kunming mice, both sexes, were randomly divided into 3 groups with 40 mice for each group： NMDA group, naloxone group and strychnine group. For each group the animals were then randomly divided into four subgroups （n=10）, one served as control and the other three as experimental subgroups. In NMDA group the subgroups were treated as follows： animals in control subgroup received intrathecal injection of artificial cerebrospinal fluid （aSCF）, and the animals in the other three subgroups were injected with 25ng, 50ng and 75ng of NMDA, respectively. For naloxone group, the mice in control subgroup received intraperitoneal injection of normal saline （NS）, and the mice in the other three subgroups were injected with 1mg/kg, 2mg/kg and 4mg/kg of naloxone, respectively. For strychnine group the mice in control subgroup received intraperitoneal injection of NS, and the mice in the other three subgroups were injected with 0.5mg/kg, 1mg/kg and 2mg/kg of strychnine, respectively. All the mice were intraperitoneally injected with thiopental sodium （50mg/kg）, then immediately injected with corresponding drug 1 minute after disappearance of righting reflex. The sleeping time of all the mice was recorded. Results NMDA threw no obvious effect on the sleeping time of the mice injected with thiopental sodium （P〉0.05）, while strychnine and naloxone obviously lengthened the sleeping time of the mice injected with thiopental sodium （P〈0.01）. Conclusion NMDA receptor is not obviously related with hypnotic effect of thiopental sodium, while both glycine receptor and opioid receptor can mediate hypnotic effect of thiopental sodium.

关 键 词：硫喷妥钠 催眠术 受体 N-甲基-D-天冬氨酸 受体 甘氨酸 受体 阿片样 

分 类 号：R614.24[医药卫生—麻醉学]