N-乙酰半胱氨酸对糖尿病大鼠心肌脂联素及其受体表达的影响  

Effects of N-acetylcysteine on the expression of cardiac adiponectin and its receptors in diabetic rats

作　　者：郭志新[1] 郑彩红[1] 魏萍[1] 秦志宏[1] 孙英姿[1] 吴苏豫[1] 

机构地区：[1]山西医科大学第二医院内分泌科,太原030001

出　　处：《解放军医学杂志》2008年第4期428-430,共3页Medical Journal of Chinese People's Liberation Army

基　　金：山西医科大学第二医院博士启动基金项目(20040104)

摘　　要：目的探讨N-乙酰半胱氨酸(NAC)对链脲佐菌素(STZ)诱导的糖尿病大鼠心肌脂联素及其受体表达的影响。方法32只雄性Wistar大鼠随机分为正常对照组(C组)、正常对照NAC治疗组(CT组),糖尿病组(D组)和糖尿病NAC治疗组(DT组),每组8只。观察8周后,用Western blot检测各组心肌脂联素受体、AMP激活蛋白激酶-α(AMPK-α)、磷酸化AMPK-α、葡萄糖转运子4(GlUT4)蛋白表达。用放射免疫法检测血浆和心肌脂联素水平。用酶免(EIA)法测定血浆和心肌游离15-F2t-异前列烷水平。结果与C组比较,D组糖尿病大鼠8周时出现心室重/体重和心肌细胞横截面积增加(P<0.05),血浆和心肌游离15-F2t-异前列烷水平升高(P<0.05),血浆脂联素水平降低(P<0.05),心肌脂联素受体1(AdipoR1)蛋白表达升高(P<0.05),心肌AMPK-α磷酸化水平和GLUT4蛋白表达降低(P<0.05),心肌脂联素及其受体2(AdipoR2)无变化。与D组比较,DT组糖尿病大鼠NAC治疗8周后心室重/体重和心肌细胞横截面积降低(P<0.05),血浆和心肌游离15-F2t-异前列烷水平下降(P<0.05),血浆和心肌脂联素水平、心肌脂联素受体、AMPK-α磷酸化和GLUT4水平无变化。结论STZ糖尿病大鼠心肌氧化应激水平增高,血浆脂联素水平降低,心肌Adi-poR1蛋白表达增高。NAC能降低糖尿病大鼠氧化应激水平,但对心肌脂联素及其受体表达无影响。Objective To investigate the effects of N-acetylcysteine （NAC） on the expression of cardiac adiponectin and its receptors in streptozotocin-induced diabetic rats. Methods Thirty-two male Wistar rats were randomly divided into four groups, with 8 in each： untreated control group （C group）, NAC-treatment group （CT group）, diabetic untreated group （D group） and diabetic NAC-treatment group （DT group）. After 8 weeks, plasma glucose and insulin, and cardiomyocyte cross sectional area were measured. Cardiac protein expression of adiponectin receptor 1 and 2 （adipoR1 and adipoR2）, AMP-activated protein kinase α （AMPKα）, phosphorylation of AMP-activated protein kinase α-subunits and glucose transporter 4 （GluT4） were determined by Western blotting. Plasma and myocardial adiponectin levels were measured by radioimmunoassay. Plasma and myocardial free 15-F2t-isoprostane levels were assayed by enzyme immunoassay. Results Compared with C group, the ratio of ventricular weight to body weight and cardiomyocyte cross-sectional area, plasma and levels of free 15-F2t-isoprostane in myocardium and the protein expression of myocardial adipoR1 increased significantly in diabetic rats （D group） （P〈0.05）, with decrease in phosphorylation of myocardial AMPK-α and protein expression of myocardial GluT4 （P〈0.05）. There were no differences in the cardiac adiponectin level and the cardiac protein expression of adipoR2 between control and diabetic rats. Eight weeks after treatment with NAC, the ratio of ventricular weight to body weight, cardiomyocyte cross-sectional area and plasma and myocardial levels of free 15-F2t-isoprostane were decreased, but plasma and myocardial adiponectin levels, myocardial levels of adiponectin receptors and phosphorylation of AMPK-α and GluT4 did not change in diabetic rats （P〈0.05）. Conclusion Myocardial oxidative stress levels were increased in STZ-induced diabetic rats. Plasma adiponectin level was decreased, but the protein expression of myocardi

关 键 词：N-乙酰半胱氨酸 糖尿病 脂联素 心肌 

分 类 号：R587.1[医药卫生—内分泌]