延胡索乙素解离常数的测定及其对分离检测影响的考察  

Determine the dissociation constants of tetrahydropalmatine and investigate the effection of its separation in medicine

作　　者：方海红[1] 朱圣生[1] 张洁[1] 吴有根[1] 魏慧珍 胡俊兴[3] 

机构地区：[1]江西中医学院,南昌330006 [2]中药固体制剂制造技术国家工程中心,南昌330006 [3]江西中医药高等专科学校,抚州344000

出　　处：《江西中医学院学报》2008年第1期91-93,共3页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：国家十一五科技支撑计划资助项目(2006BAI08B03-05)

摘　　要：目的:测定延胡索乙素的解离常数(pKa),考察流动相pH对其保留行为、柱效影响与pKa值的相关性,为延胡索乙素的分离检测提供指导。方法:以对硝基酚为指示剂,用分光光度指示剂法测定延胡索乙素的解离常数;采用高效液相色谱法,考察流动相pH对延胡索乙素保留行为、柱效的影响,优化延胡索中延胡索乙素分离条件。结果:延胡索乙素的值为(6.49±0.07)(25±0.5)℃;流动相pH对延胡索乙素保留行为的影响与其pKa值有明显的相关性,流动相为乙腈-0.1%磷酸(三乙胺调pH6)(45:55)可较好的分离检测延胡索中的延胡索乙素。结论:酸碱性药物解离常数的测定,对其分离条件的选择有重要的指导意义。Objective： Determine the dissociation constants of tetrahydropalmatine, investigate the correlation pKa with the effection of retention behavior and column efficiency by pH of mobile phase, and it can offer direction to separate tetrahydropalmatine in medicine. Method ：4-nitrophenol as an indicator, the dissociation constant of tetrahydropalmatine was determined by the spectrophotometrie indicator method. Investigate the effection of retention behavior and column efficiency by pH of mobile phase by HPLC. Optimize the separation and detection of tetrahydropalmatine in Rhizoma Corydalis. Result： of tetrahydropalmatine was （6.49 ± 0.07 at） （25 ± 0. 5 ） ℃. And pKa obviously correlate with the effection of retention behavior by pH of mobile phase. When mobile phase pH was 6.0, tetrahydropalmatine in Rhizoma Corydalis can be separated and detected very well. Conclusion The determination of dissociation constants of medicine was significance to guide the optimization condition of separation and detection.

关 键 词：延胡索乙素 解离常数 分光光度指示剂法 保留行为 柱效 

分 类 号：R284.1[医药卫生—中药学]