液相色谱-质谱联用法测定中药抗风湿制剂中非法掺入吡罗昔康的研究  

Determination of piroxicam illegally mixed into traditional Chinese medicine anti-rheumatism preparations by the liquid chromatography-quadrupole mass spectrometry method

作　　者：邱颖姮[1] 王铁杰[1] 杨敏[1] 李军[1] 

机构地区：[1]深圳市药品检验所,深圳518029

出　　处：《中国新药杂志》2008年第6期506-509,共4页Chinese Journal of New Drugs

摘　　要：目的:建立中药抗风湿制剂中非法掺入吡罗昔康专属性的检测方法。方法:采用液相色谱-串联四极杆质谱联用法。选用SunFireTMC18柱,以0.01 mol.L-1乙酸铵水溶液-乙腈(80∶20)为流动相,检测波长334 nm,流速为0.2 mL.min-1。对中药抗风湿制剂的提取液进行液相色谱-串联四极杆质谱分析。通过与对照品的色谱及质谱行为相比较,对中药抗风湿制剂中非法掺入的吡罗昔康进行定性鉴别。结果:在4种受试中药抗风湿制剂中,1种被检测到掺有吡罗昔康。结论:该方法选择性强,灵敏度高,可作为分析检测中药抗风湿制剂中非法掺入吡罗昔康的有效方法。Objective：To develop a specific method for the identification of piroxicam illegally added into traditional Chinese medicine anti-rheumatism preparations. Methods：The liquid chromatography-quadrupole mass spectrometry method was used with SunFireTM C1s column, the mobile phase was 0.01 mol· L^-1 ammonium acetate solution-acetonitrile（80： 20）, the detecting wavelength was at 334 nm, flow rate was 0.2 mL· min^-1. After being separated by the chromatographic-mass spectrometry, the organic extraction of the traditional Chinese medicine antirheumatism preparations was analyzed. The compounds illegally added into the traditional Chinese medicine antirheumatism preparations were identified according to the chromatographic behavior and mass spectral data by comparison with those of reference substances. Results：Piroxicam was found in one among 4 formulations. Conclu- sion：The method is selective and sensitive and can be used to detect the illegal traditional Chinese medicine antirheumatism preparations.

关 键 词：中药抗风湿制剂 吡罗昔康 液相色谱-串联四极杆质谱法 

分 类 号：R927.2[医药卫生—药学] R971.1