虎眼万年青不同形态发生途径激素调控的初步研究  

STUDY ON HORMONAL CONTROL OF MORPHOGENETIC PATHWAYS IN ORNITHOGALUM CAUDATUM BY AUXINS AND CYTOKININS

作　　者：张光祥[1] 

机构地区：[1]四川师范大学生命科学技术系

出　　处：《四川师范大学学报（自然科学版）》1997年第5期73-79,共7页Journal of Sichuan Normal University（Natural Science）

摘　　要：用虎眼万年青鳞片外植体在ＭＳ培养基上实现了不同形态发生途径的激素调控．结果显示，生长素和细胞分裂素两类激素的不同组合和浓度搭配都是影响形态发生途径的关键因素，甚至前者更为重要．就最佳激素组合和浓度搭配而言，ＮＡＡ０．５ｍｇ／Ｌ＋ＢＡ０．５ｍｇ／Ｌ和ＮＡＡ０．５ｍｇ／Ｌ＋ＫＴ０．５ｍｇ／Ｌ分别诱导体细胞胚和不定芽直接发生；２，４－Ｄ４．０ｍｇ／Ｌ＋ＢＡ１．０ｍｇ／Ｌ诱导愈伤组织；２，４－Ｄ０．１ｍｇ／Ｌ＋ＢＡ２．０ｍｇ／Ｌ诱导无根苗从基部分化体细胞胚．所有无根苗都能在含ＮＡＡ２．０ｍｇ／Ｌ的１２ＭＳ培养基上生根．本文结合前人的研究对体细胞胚胎发生和器官发生的判别标准、异常体细胞胚的形成等问题作了必要的讨论．Various morphogenetic pathways in Ornithogalum caudatum (Ait.) are regulated by manipulating the combination and ratio of auxins and cytokinins on MS medium. The results showe it is more important to select the optimal combination and ratio of auxins and cytokinins than to evaluate the effectiveness of every hormone one by one. For the bulb scale of O. caudatum, NAA 0.5mg/L+BA 0.5mg/L is optimal to induce direct somatic embryogenesis; NAA 0.5mg/L+KT 0.5mg/L to direct shoot formation; 2,4 D 4.0 mg/L+BA1.0mg/L to callus; 2,4-D 0.1mg/L+BA 2.0mg/L to embryogenesis from the base of rootless seedling. Formation of abnormal somatic embryos and criteria of differentiating embryogenesis and shoot formation are discussed in the present paper.

关 键 词：形态发生途径 激素调控 愈伤组织 虎眼万年青 

分 类 号：Q949.718.2[生物学—植物学] S682.14[农业科学—观赏园艺]