三个候选基因多态性与糖皮质激素性骨质疏松症关联的研究  

Relationship between the single nucleotide polymorphisms in three candidate genes and the glucocorticoid-induced osteoporosis

作　　者：隋满姝[1] 那士平[1] 解汝娟[1] 刘睿婵[1] 贾西贝[1] 

机构地区：[1]哈尔滨医科大学附属第一医院肾内科,哈尔滨150001

出　　处：《中国血液净化》2008年第2期61-64,共4页Chinese Journal of Blood Purification

基　　金：黑龙江省教育厅资助项目(11521123)

摘　　要：目的探讨护骨素(OPG)A163G、瘦素受体(LEPR)Gln223Arg和过氧化物酶体增殖物激活受体γ(PPARγ)C161T3个单核苷酸多态性(SNPs)与糖皮质激素性骨质疏松症(GIO)的关系。方法选择哈尔滨医科大学附属第一医院肾内科患者采用限制性片断长度多态性法,在208例健康对照组、168例非GIO组和104例GIO组中分析A163G、Gln223Arg和C161T多态性的基因型分布;应用双能X线骨密度仪(DEXA)测定股骨、腰椎等部位的骨密度。结果在单基因研究中,GIO组患者与健康对照组比较,A163G和C161T基因型和等位基因频率差异有显著意义(P<0.05);采用多基因联合分析,OPGGG纯合子中,PPARγTT纯合子GIO患病率显著增高(OR=2.04,95%CI1.26～3.67,P=0.02)。结论OPG A163G和PPAR γC161T多态性可能与GIO的发病相关;在GIO人群中,OPG A163G和PPAR γC161T多态可能有协同作用。Objective To explore the relationship between the 3 single nucleotide polymorphisms （SNPs） in three candidate genes, i.e., A163G in osteoprotegerin （OPG）, Gln223Arg in leptin receptor （LEPR） and C161T in peroxisome proliferator-activated receptor （PPARγ）, and the glucocorticoid-induced osteoporosis （GIO） in Chinese population. Methods We used the method of polymerase chain reaction-restriction fragment length polymorphisms （PCR-RFLP） to identify the SNPs of A163G in OPG, Gln223Arg in LEPR and C161T in PPAR in 208 normal controls, 168 cases without GIO and 104 cases with GIO. Bone mineral density at lumbar spine and femoral neck was measured by using dual-energy X-ray absorption-metry （DEXA）. Results By analysis of GIO with the respective SNPs, we obtained that the incidence of A163G in OPG and C161T in PPARγ as well as their related allele frequencies were significantly different between GIO cases and normal controls （P〈0.05）. When GIO was evaluated with the 3 SNPs, we found that the genotype rate of homologous GG at 163 in OPG in association with TT at 161 in PPARγ was significantly high in GIO cases （OR=2.04, 95%CI=1.26～3.67, P=0.02）. Conclusion The SNPs of A163G in OPG and C161T in PPARγ may correlate with the glucocorticoid-induced osteoporosis in Chinese population, and these 2 SNPs may synergically interact in glucocorticoid-induced osteoporosis.

关 键 词：基因 多态性 单核苷酸 糖皮质激素 骨密度 

分 类 号：R692[医药卫生—泌尿科学]