大鼠模拟经前期综合征肝气逆证不同脑区E_2受体、P受体基因表达mRNA水平变化  

Estrogen and Progesterone Receptors' Mrna Level Expression Variation in Different Brain Areas of Rat Models

作　　者：马月香[1] 乔明琦[1] 张惠云[1] 

机构地区：[1]山东中医药大学,济南250014

出　　处：《世界科学技术-中医药现代化》2008年第1期68-71,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金重大课题(90209003):PMS肝气逆证受体基因表达及其非线性动力学特征

摘　　要：目的:探讨肝主疏泄与雌二醇受体(ER)、孕酮受体(PR)基因表达的关系。方法:以肝失疏泄所导致的始发证侯肝气逆证为切入点,运用RT-PCR方法,对肝失疏泄产生肝气逆证时的主要改变指标ER、PR的基因表达在中枢不同脑区(下丘脑、边缘叶)mRNA水平上的变化进行探讨。结果:在肝失疏泄产生肝气逆证时ER、PR的活性皆明显下降,且在下丘脑中以PR的活性下降为主,在边缘叶中以ER的活性下降为主。结论:中枢神经系统下丘脑中PR的活性下降和边缘叶中ER的活性下降,可做为诊断肝气逆证的微观指标之一。This paper discusses the relationships between liver controlling dredging and both Estrogen and Progesterone receptors＇ expression. Authors observe both Estrogen and Progesterone receptors＇ expression variations across different CNS areas （lower part of Thalamus, Border leaf）, using molecular biological technique. They found that when liver controlling dredging leads to a Liver-qi invasion, gene expressions of both Estrogen and Progesterone receptors would be reduced in a noticeable manner. Progesterone receptor has a reduced gene expression mainly in the lower part of Thalamus, while Estrogen receptor mainly in the Border leaf. It is concluded that the reduced gene expression of Progesterone and Estrogen receptors can be used as an indicator for diagnosing Liver-qi Invasion.

关 键 词：肝气逆证 雌二醇受体 孕酮受体 基因表达 

分 类 号：R977[医药卫生—药品] R995[医药卫生—药学]