BAPBE的合成及其铁螯合物对蛋白质的切断作用  

Synthesis of BAPBE and Cleavage of Proteins via Protein-BAPBE-Fe^(2+) Conjugates

作　　者：厉廷有[1] 曹红花 王宗睦[1] 

机构地区：[1]吉林大学分子生物系,长春130023

出　　处：《吉林大学自然科学学报》1997年第4期98-102,共5页Acta Scientiarum Naturalium Universitatis Jilinensis

基　　金：国家自然科学基金

摘　　要：设计、合成了一种新的蛋白质特异性氧化断裂试剂BAPBE，用它共价修饰BSA的唯一疏基．修饰物与Fe2+螯合，在pH7．0、25℃下用H2O2及抗坏血酸钠处理，使BSA发生2种方式的断裂，产生4种BSA片段，但在相同条件下巯基枯草杆菌蛋白酶未被切断．A new protein cleaving reagent, 1-[4-(4-bromoacetylamidophenoxy)benzyl]EDTA (BAPBE), was synthesized from tyrosin in 7 steps. Only the mercapto group of BSA was alkylated with BAPBE and then the resulted modification, BSA-BAPBE, was treated with a solution containing Fe2+ and H2O2/ascorbic acid successively. It was found that BSA was cleft specifically in four fragments, their Mw were 5.7×103, 3.6×103, 3.0×103 and ≤103 respectively. Under the same condition, however, the thiol-subtilisin-BAPBE conjugate was unaffected.

关 键 词：蛋白质 特异性断裂 BAPBE 铁螯合物 

分 类 号：Q51[生物学—生物化学] O629.73[理学—有机化学]