炎性多肽Daintain促进乳腺癌细胞MCF-7增殖  

Daintain,an Inflammatory Polypeptide,Promotes the Proliferation of MCF-7 Cells

作　　者：刘收[1] 陈正望[1] 

机构地区：[1]华中科技大学生命科学与技术学院生物物理学与生物化学研究所,武汉市430074

出　　处：《医学分子生物学杂志》2008年第2期129-132,140,共5页Journal of Medical Molecular Biology

摘　　要：目的构建重组质粒pcDNA-Daintain(pcDNA-DT),并研究pcDNA-DT对乳腺癌细胞MCF-7增殖的影响,为进一步了解Daintain与肿瘤发生、发展的关系奠定基础。方法采用分子克隆技术,将Dain-tain基因克隆到pcDNA表达载体上,用脂质体法导入到乳腺癌细胞MCF-7中;RT-PCR和Western印迹方法检测Daintain基因的表达;通过细胞计数和MTT方法检测Daintain过表达以及外源Daintain对细胞增殖的影响。结果重组质粒pcDNA-DT经酶切和测序鉴定,其目的片段大小,插入位点和核苷酸序列完全正确;转染细胞中Daintain的表达明显多于对照细胞;Daintain过表达能显著促进MCF-7增殖;当外源Daintain浓度大于1μg/ml时对MCF-7细胞有明显的促增殖作用。结论Daintain对乳腺癌细胞MCF-7的增殖有促增殖作用。Objective To construct recombinant plasmid pcDNA-DT and study the effect of daintain on the proliferation of MCF-7, to provide a basis for further research on the association of daintain with the development of cancers. Methods Daintain gene was cloned into pcDNA by molecular cloning and transfected into breast cancer cells by liposome. RT-PCR and Western blotting were used to determine the expression of daintain. The effect of daintain overexpression and exogenous daintain on the proliferation of MCF were examined by counting cells with hemocytometer and by MTI＇, respectively. Results Enzyme digestion and DNA sequencing showed that the target gene was cloned into expression vector. RT-PCR and Western blotting demonstrated that the expression of daintain in the MCF-7 transfected with pcDNA-DT was strouger than that of cells with pcDNA alone. It was further found that daintain overexpression promoted the proliferation of MCF-7. Similarly, ex- ogenous daintain increased the growth rate of MCF-7 when its concentration was over 1 μg/ ml. Conclusion Daintain can promote the proliferation of MCF-7 cells by either endogenous or exogenous pathway.

关 键 词：daintain MCF-7 增殖 

分 类 号：R341.2[医药卫生—基础医学]