调中颗粒对混合反流性食管炎大鼠的疗效  

Effects of Tiaozhong granule on experimental mixed reflux esophagitis in rats

作　　者：时昭红[1] 张介眉[1] 郝建军[1] 冯云霞[1] 陈洲[2] 

机构地区：[1]武汉市第一医院消化内科,湖北省武汉市430022 [2]湖北中医学院中医系,湖北省武汉市430061

出　　处：《世界华人消化杂志》2008年第7期695-700,共6页World Chinese Journal of Digestology

基　　金：武汉市卫生局临床重点学科研究项目;武卫No.[2003]228~~

摘　　要：目的:研究调中颗粒对混合反流性食管炎大鼠食管黏膜血管活性肠肽(VIP)、P物质(SP)的水平及血浆中脂质过氧化物(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)活性的影响.方法:将大鼠随机分成5组,调中颗粒组(A组)、西沙必利组(B组)、半夏泻心汤组(C组)、正常对照组(D组)、空白模型组(E组),用食管十二指肠端侧吻合术法制备混合反流性食管炎大鼠模型,于给药2wk后处死大鼠,HE染色观察病理改变,免疫组织化学技术检测大鼠胃黏膜VIP和SP的水平,比色法检测血浆中SOD、MDA和GSH-PX的活性.结果:与E组比较,A、B、C组光镜下食管黏膜组织有不同程度的修复,平均R值分别为0.8532、0.5521、0.7865,差异显著(P<0.05),其VIP和SP表达明显增强(VIP:113.61±11.36,118.74±12.81,107.33±15.95vs91.05±10.66,P<0.05;SP:132.24±10.51,140.86±11.63,133.54±14.02vs113.73±16.37,P<0.05);MDA含量降低(4.11±0.37,4.64±0.60,4.29±0.29vs5.77±0.16,P<0.05)、SOD和GSH-PX的活性提高(SOD:431.94±21.87,402.98±36.59,384.66±46.02vs345.02±24.11,P<0.05;GSH-PX:135.83±3.71,118.26±6.34,122.78±4.76vs112.77±6.20,P<0.05).结论:调中颗粒可能通过SP和VIP表达增强来调节胃肠运动从而降低MDA的含量,提高SOD和GSH-PX的活性,抑制反流性食管炎的发展,促进黏膜的修复,从而起到抑制胃肠内容物的反流和反流性食管炎的发展的作用。AIM： To study the influence of Tiaozhong granuls （TZG） on the vasoactive intestinal peptide （VIP）, substance P （SP）, superoxide dismutase （SOD）, malondialdehyde （MDA） and glutathione perioxidase （GSH-PX） in rats with experimental mixed reflux esophagitis. METHODS： The rats were randomly divided into five groups： model group （E）, normal group （D）, TZG group （A）, Banxia Xiexin decoction group （C） and cisapride group （B）. Then esophagoduodenal end-to-side anastomosis was used to make rat model of mixed reflux esophagitis. The histopathological examination （HE staining） was used to observe the changes of esophageal mucosa. The contents of SP and VIP in esophageal tissues were detected 2 wk after drugadministration by immunohistochemical technique, and spectrophotometric method was used to detect the activity of SOD, MDA and GSH-PX. RESULTS： In comparison with those in group E, the contents of VIP and SP were markedly increased in groups A, B and C （VIP： 113.61 ± 11.36, 118.74 ± 12.81, 107.33 ± 15.95 vs 91.05 ± 10.66, P 〈 0.05; SP： 132.24 ± 10.51, 140.86 ± 11.63, 133.54 ± 14.02 vs 113.73 ± 16.37, P 〈 0.05）, and the esophageal mucosa under light microscope were also repaired to some extent, with R values of 0.8532, 0.5521 and 0.7865, respectively; meanwhile, MDA content was decreased （4.11 ± 0.37, 4.64 ± 0.60, 4.29 ± 0.29 vs 5.77 ± 0.16, P 〈 0.05）, and SOD and GSH-PX activity were enhanced （SOD： 431.94 ± 21.87, 402.98 ± 36.59, 384.66 ± 46.02 vs 345.02 ± 24.11, P 〈 0.05; GSH-PX： 135.83 ± 3.71,118.26 ± 6.34, 122.78 ± 4.76 vs 112.77 ± 6.20, P 〈 0.05）. CONCLUSION： TZG can treat mixed reflux esophagifis by increasing the expression of VIP and SP, the activity of SOD and GSH-PX and decreasing MDA.

关 键 词：反流性食管炎 调中颗粒 氧自由基 脑肠肽 

分 类 号：R285.5[医药卫生—中药学]